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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 10-16.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

水稻种子萌发过程中胚蛋白质差异表达分析

许丽璇,蔡建秀   

  • 收稿日期:2009-11-02 修回日期:2009-12-02 出版日期:2010-03-20 发布日期:2010-03-20
  • 基金资助:

    福建省教育厅项目“水稻穗上发芽的生理生化及分子机理研究”

Differential Proteomic and Mass Spectrometric Analysis of embryo buds During Seed Germination in Rice(Oryza sativa L.)

  • Received:2009-11-02 Revised:2009-12-02 Online:2010-03-20 Published:2010-03-20

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摘要：

采用IEF-SDS-PAGE双向电泳技术，对金稻1号水稻种子未萌发和萌发1-4d胚进行蛋白质分离。发现金稻1号水稻种子未萌发和萌发1-4d胚在PDQuest图像分析软件可识别的蛋白质点约215、201、187、162、132个，其中表达量变化2.5倍以上的蛋白质点有22个。选取表达量变化2.5倍以上的12个差异蛋白质点进行胶内酶解，并进行肽质量指纹图谱及其生物信息分析，初步鉴定出6个蛋白质，分别为硫氧还蛋白； 热休克蛋白；ATP合酶；LEA蛋白12；核苷二磷酸激酶；乙二醛酶。对这些蛋白质在水稻种子萌发过程中的作用进行了讨论。

关键词: 苹果组培苗, 苹果组培苗, 种质保存, 延缓生长, 生长调节剂

Abstract:

IEF- SDS- PAGE two- dimensional gel electrophoresis (2- DE) technology was used to separated proteins from ungerminated seed and embryo buds of germinated seed at specific stages of 1 d、2d、3d、4d in rice(Oryza sativa L.)Jindao 1. The results showed that on the 2-DE gels stained by coomassie brilliant blue，PDQuest image software detected about 215、201、187、162、132 protein spots， of which 22 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 1 d、2d、3d、4d in rice(Oryza sativa L.)Jindao 1. These 12 of 22 protein spots treated by tryptic in-gel digestion were characterized by matrix-assisted laser -desorption／ionization time of flight mass spectrometry (MALD I-TOF -MS) and bioinformatics analysis,peptidemass fingerprintings of all were obtained，of which 6 differential proteins were elementarily identified as thioredoxin peroxidase，heat shock protein, ATP synthase, LEA protein 12, nucleoside diphosphate kinaseⅠ(NDK Ⅰ)(NDP kinase Ⅰ)(NDPK Ⅰ)and glyoxalase I respectively. The potential functions of these proteins during seed germination were discussed．