植物生长调节剂对百香果的影响

doi:10.11924/j.issn.1000-6850.casb2021-0063

摘要/Abstract

摘要：

为研究植物生长调节剂对百香果氮代谢、防御系统与果实品质的关系,在百香果幼果期、果实膨大期、着色期喷施2,4-二氯苯氧乙酸(B)、氨基寡糖素(C)、24-表芸苔素内酯(D),通过测定氮代谢相关产物及其关键酶活力、酶促防御系统和非酶促防御系统中相关物质、果实外观品质和内在品质,初步探究植物生长调节剂对百香果的影响。结果表明,B、C、D处理百香果叶片GS较对照处理(A)升高100.74%、25.92%、62.53%,可溶性蛋白含量较A处理降低54.19%、47.26%、10.11%;B处理抗坏血酸(ASA)、谷胱甘肽(GSH)含量最高,过氧化氢酶(CAT)、过氧化物酶(POD)活力分别提高31.99%、79.44%;B、C、D处理后百香果果实可溶性固形物较A处理升高5.82%、3.53%、6.03%,有机酸较A处理升高4.87%、8.97%、10.58%。3种植物生长调节剂通过影响百香果氮代谢相关酶活力和产物、防御系统影响果实品质,芸苔素处理百香果果实可溶性固形物、有机酸、可溶性糖、Vc含量均较高,品质最优。

关键词: 百香果, 植物生长调节剂, 氮代谢, 防御系统, 果实品质

Abstract:

The aim of this paper is to investigate the effect of plant growth regulators on Passiflora edulia nitrogen metabolism and defense system, and on their relationship with the fruit quality. We sprayed 2,4-dichlorophenoxyacetic acid (B) and amino-oligosaccharin (C) and 24- epbrassinolide (D) during the fruitlet period, fruit expanding period and coloring period, and measured the nitrogen metabolism related products and key enzyme activities, enzymatic defense system and non-enzymatic defense system related material, fruit appearance quality and intrinsic quality. The results showed that compared with control treatment (A), GS in the leaves of treatment B, C and D increased by 100.74%, 25.92% and 62.53%, respectively, and soluble protein content decreased by 54.19%, 47.26% and 10.11%, respectively. The contents of ascorbic acid (ASA) and glutathione (GSH) in treatment B were the highest, and the activities of catalase (CAT) and peroxidase (POD) increased by 31.99% and 79.44%, respectively. After treatment B, C and D, the soluble solids of Passiflora edulia fruits increased by 5.82%, 3.53% and 6.03%, and the organic acids increased by 4.87%, 8.97% and 10.58% compared with treatment A. In conclusion, the three plant growth regulators could affect fruit quality by affecting the enzymes activities and products related to nitrogen metabolism and defense system, and fruits treated by 24- ep-brassinolide have relatively high contents of soluble solids, organic acids, soluble sugars and Vc, and the best quality.

Key words: Passiflora edulia, plant growth regulators, nitrogen metabolism, defense system, fruit quality

中图分类号:

S667.9

杨琴, 俞露, 赵芷, 池继焰, 谭书明. 植物生长调节剂对百香果的影响[J]. 中国农学通报, 2021, 37(19): 58-64.

Yang Qin, Yu Lu, Zhao Zhi, Chi Jiyan, Tan Shuming. Effects of Plant Growth Regulators on Passiflora edulia[J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 58-64.

图/表 8

参考文献 30

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处理	NR/(U/g)	GS/[U/(g·h)]	GPT/[μmol/(g·30 min)]	GOT/[μmol/(g·30 min)]
A	9.17±0.29a	0.451±0.017d	30.84±1.07a	21.49±0.37a
B	6.85±0.53b	0.906±0.007a	33.42±0.61a	21.15±0.34ab
C	6.71±0.67b	0.568±0.003c	26.15±0.36b	22.05±0.18a
D	6.45±0.82b	0.733±0.003b	31.29±1.01a	20.83±0.26b

处理	NR/(U/g)	GS/[U/(g·h)]	GPT/[μmol/(g·30 min)]	GOT/[μmol/(g·30 min)]
A	9.17±0.29a	0.451±0.017d	30.84±1.07a	21.49±0.37a
B	6.85±0.53b	0.906±0.007a	33.42±0.61a	21.15±0.34ab
C	6.71±0.67b	0.568±0.003c	26.15±0.36b	22.05±0.18a
D	6.45±0.82b	0.733±0.003b	31.29±1.01a	20.83±0.26b

处理	MDA含量/(μmol/g)	GSH含量/(mmol/g)	ASA含量/(mg/g)
A	6.47±1.4a	4.387±0.04a	521.26±21.44a
B	3.41±0.6b	4.395±0.26a	563.66±12.68a
C	6.35±1.13a	3.673±0.11b	433.15±8.97b
D	6.59±0.82a	3.970±0.06ab	549.83±32.32a

处理	MDA含量/(μmol/g)	GSH含量/(mmol/g)	ASA含量/(mg/g)
A	6.47±1.4a	4.387±0.04a	521.26±21.44a
B	3.41±0.6b	4.395±0.26a	563.66±12.68a
C	6.35±1.13a	3.673±0.11b	433.15±8.97b
D	6.59±0.82a	3.970±0.06ab	549.83±32.32a

处理	单果质量/g	纵径/mm	横径/mm	果形指数
A	50.89±11.5a	60.6±2.83a	55.53±2.54a	0.92±0.05a
B	51.3±10.21a	59.95±4.12a	54.67±3.88a	0.91±0.04a
C	54.12±12.58a	51.04±12.69a	50.89±11.5a	0.93±0.04a
D	51.04±12.69a	61.01±4.98a	57.31±4.2a	0.94±0.03a