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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (6): 73-76.

• 农学 农业基础科学 • 上一篇    下一篇

闽引圆叶决明两个辐射变异新品系的若干特性研究

郑向丽,叶花兰,徐国忠   

  • 收稿日期:2009-11-02 修回日期:2009-11-25 出版日期:2010-03-20 发布日期:2010-03-20
  • 基金资助:

    超高产狼尾草属、决明属牧草新品种选育及产业化示范;红壤丘陵开发地生态恢复与综合治理开发模式示范;决明属牧草耐铝毒基因型筛选及筛选指标的研究;山地红壤－豆科牧草（圆叶决明)系统中氮素迁移转化规律的研究

Study on Agronomic Characters of two Changed—plants of Chamaecrista rotundifolia cv.MINYIN by Irradiation

  • Received:2009-11-02 Revised:2009-11-25 Online:2010-03-20 Published:2010-03-20

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摘要：

利用 60Coγ射线辐射闽引圆叶决明86134牧草种子，通过M1-M3代田间试验观察和筛选，筛选到两个86134的辐射变异后代，进行M4、M5代田间小区品比试验，结果表明：2个变异品系的茎叶比大幅度下降，其生物量分别增长39.26%和38.04%，达极显著水平，86134-32-3的粗脂肪含量极显著增长。RAPD分析表明，辐射后代与原种的DNA指纹图谱存在明显差异。

关键词: 猪, 猪, MEF2a基因, 肌肉发生, Real-time PCR

Abstract:

The seeds of Chamaecrista rotundifolia cv.MINYIN pasturages were irradiated by 60Co-γ. Based on the observations of M1, M2, M3 generation in fiel experiment, two plants with bigger variations in agronomic character were elected through M4,M5 observation. The results showed that the ratio of stem and leaf of two changed-plants decreased, The biomasses have increased 39.26％ and 38.04％ at 0.01 level．Crude fattiness of mutant 86134-32-3 increased at 0.01 level. On the basis of analysis by using random amplified polymorphic DNA （RAPD） markers, it was found that there were obvious differences in DNA fingerprinting between the progenies after irradiation by 60Co-γ and their parents.