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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (4): 30-32.

所属专题： 生物技术； 马铃薯

• 生物技术科学 • 上一篇    下一篇

利用改良CTAB法提取木薯基因组DNA

闫庆祥1,2,黄东益2,李开绵1,叶剑秋1   

  • 收稿日期:2009-11-25 修回日期:2009-12-02 出版日期:2010-02-20 发布日期:2010-02-20
  • 基金资助:

    中央级公益性科研院所基本科研业务费专项资金资助(中国热带农业科学院热带作物品种资源研究所)资助项目;公益性行业科研专项项目资助

Genomic DNA Extraction in Cassava by Modified CTAB Method

  • Received:2009-11-25 Revised:2009-12-02 Online:2010-02-20 Published:2010-02-20

PDF (PC)

1811

摘要：

摘要：采用改良CTAB法提取木薯基因组DNA，该方法提取的基因组DNA琼脂糖凝胶电泳条带清晰，无拖尾现象。结果表明，DNA浓度在710.0 ng/μl～967.5 ng/μl 之间，OD260/OD280在1.73～1.92之间，该方法具有简便、快速、高效等特点，所提取的基因组DNA质量和纯度较高，适用于进一步的SSR、ISSR等分子标记分析。

关键词: NDVI, NDVI, 傅利叶变换, 地表覆被分类

Abstract:

Abstract: In this paper，genomic DNA extraction in cassava by modified CTAB method，the extracted products had the clear band and without tailing bands which could be observed through agar gel electrophoresis. The results show that the concentrations were between 710.0 ng/μl～967.5 ng/μl，and the values of OD260/OD280 were between 1.73～1.92. The modified CTAB method had characteristics of simple、rapid and effective，the genomic DNA extracted had high quality and purity，it could be used for SSR、 ISSR and other molecular markers.