欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (7): 1-5.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

特布他林人工抗原的合成及鼠源多克隆抗血清的制备

职爱民1,王磊1,2,刘庆堂1,胡骁飞1,杨苏贞1,柴书军1,邓瑞广1,张改平1   

  • 收稿日期:2009-12-01 修回日期:2009-12-22 出版日期:2010-04-05 发布日期:2010-04-05
  • 基金资助:

    “十一五”国家科技支撑计划“畜禽及其制品安全生产的质量控制技术研究”

Synthesis of Terbutaline artificial antigen and development of its mice polyclonal antiserum

  • Received:2009-12-01 Revised:2009-12-22 Online:2010-04-05 Published:2010-04-05

PDF (PC)

861

摘要：

合成并鉴定特布他林（Terbutaline，TBL）人工抗原，通过动物免疫生产亲和力高、特异性好的鼠源TBL多克隆抗血清。分别通过1,4丁二醚法和EDC法将特布他林偶联于载体蛋白BSA和OVA上，分别合成免疫抗原BSA-TBL和包被抗原OVA-TBL，并采用紫外分光光度法和SDS-PAGE进行鉴定，通过动物免疫试验获得鼠源多抗血清，并使用ELISA和阻断ELISA的方法对多抗血清进行了鉴定。结果表明半抗原TBL分别和载体蛋白BSA及OVA偶联成功；受免6只小鼠效价均达1×10-4以上，且1号小鼠多抗血清抗TBL的IC50为55.88 ng/mL。本试验成功合成了TBL人工抗原，并生产了高效价、高敏感性的鼠源多克隆抗血清，为TBL单克隆抗体制备及其快速检测试剂的研制奠定了坚实的基础。

关键词: 播期, 播期, 蛋白含量, 油分含量, 平均荚鲜重, 平均粒鲜重

Abstract:

The goal of this study was to synthesize artificial antigen of TBLaterol and obtain its mice polyclonal antiserum. Artificial antigen BSA-TBL and OVA-TBL were synthesized using butane-1,4-diol diglycidyl ether and EDC by linking carrier proteins BSA and OVA to TBL. The antigen BSA-TBL and OVA-TBL was identified by ultraviolet scanning, SDS-PAGE. The mice polyclonal antiserum was identified by ELISA and blocking ELISA. The results showed that the hapten TBL was successfully linked to carrier proteins. The animal immunization results showed that six BALB/c mice indirect ELISA titer against TBL were above 1×10-4 and the IC50 of No. 1 mice was 55.88 ng/mL . The high-titer mice polyclonal antiserum has been generated. Artificial antigen BSA-TBL and OVA-TBL were synthesized and sensitivity TBL polyclonal antiserum has been generated in this study. The artificial antigen of TBL and its mice polyclonal antiserum laid a foundation base for product the monoclonal antibodies and rapid test reagent against TBL.