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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (1): 27-31.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

大蕉幼雄花易碎胚性愈伤组织长期继代培养及其植株再生

李哲   

  • 收稿日期:2009-12-04 修回日期:2009-12-23 出版日期:2010-01-05 发布日期:2010-01-05
  • 基金资助:

    国家自然科学基金项目;国际科技合作项目;海南省重点科技项目;行业科技专项;基本科研业务费专项资金项目

Long-term Subculture and Plant Regeneration from Friable and Embryogenic Callus Induced from Inflorescences of Musa paradisiaca ABB Linn.

  • Received:2009-12-04 Revised:2009-12-23 Online:2010-01-05 Published:2010-01-05

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摘要：

大蕉未成熟雄花接种到胚性愈伤组织诱导培养基中，4～5个月后可诱导出胚性愈伤组织，并可在继代培养基上长期增殖。这些继代培养了6年多的松软易碎的胚性愈伤组织转移到含有0.2 mg/L 6-BA的分化培养基中，可诱导出芽，得到了53株再生植株，这些再生植株可进一步扩大繁殖。组织学切片证明长期继代培养的愈伤组织维持了胚性的状态。

关键词: 甘薯, 甘薯, 产量, 农艺性状, 气象因子, 关联度

Abstract:

Immature male flowers of Musa paradisiaca ABB Linn. were inoculated on embryogenic callus induction media. Yellow granular calli emerged after 4–5 months were shown to be embryogenic calli. And they could be proliferated by long-term subculture. These friable and embryogenic calli had been subcultured for 6 years. After they were transplanted on differentiation medium containing 0.2 mg/L 6-BA, buds could be induced, and 53 regeneration plantlets had been obtained. These plantlets could be propagated further. Histological sections proved that the calli proliferated by long-term subculture maintained the embryogenic character.