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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 263-266.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

刺五加体细胞胚再生体系的研究

褚丽敏1,赵洪利2   

  • 收稿日期:2009-12-16 修回日期:2010-01-04 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:

    辽宁省“十一五”攻关项目:设施农业高效生产技术集成与示范,辽宁省教育厅优秀人才项目

Study on regenerate system of Acanthopanax senticosus somatic embryo

Chu Limin1, Zhao Hongli2   

  • Received:2009-12-16 Revised:2010-01-04 Online:2010-05-20 Published:2010-05-20

PDF (PC)

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摘要：

以刺五加种子为外植体，采用固体培养筛选出最优培养基，并成功地诱导出体细胞胚获得再生植株，以此来建立一套成熟稳定的刺五加体细胞胚再生体系。试验结果表明， 最适宜的胚性愈伤组织诱导培养基为1/3MS+1.0 mg?L-12,4-D,其诱导率可达50%；胚性愈伤组织增殖的最适培养基为1/3MS+1.0 mg?L-12,4-D，其增殖倍数可达4.24倍；胚性愈伤组织在不添加任何植物生长调节物质的1/3MS培养基上培养，可促进体细胞胚的形成、萌发及植株转化率。添加10 g?L-1蔗糖时，体细胞胚的发芽率达到90%，植株转化率达到97.8%。

关键词: 粮食总产量, 粮食总产量, 单产, 播种面积, 逐步回归, 辽宁省

Abstract:

Using seeds explants,an efficient plant regeneration system via somatic embryo was established for Acanthopanax senticosus.The results showed that 1/3MS+1.0 mg?L-12,4-D was the optimum medium for the embryogenic callus induction;1/3MS+1.0 mg?L-12,4-D was the optimum medium for the embryogenic callus proliferation，propagating times was 4.24.;It was increased the somatic embryo development that the embryogenic callus was transferred to 1/3MS medium with 2,4-D lacking. Furthermore, when the medium was appended 10g?L-1sucrose，the somatic embryo germination rate and plant conversion rate was 90% and 97.8% respectively.