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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 307-310.

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

培养时间对玉米灰斑病菌毒素活性的影响

王桂清1,2,张涛2,孙华2,徐秀德1,董怀玉1   

  • 收稿日期:2009-12-21 修回日期:2010-01-14 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    山东省中青年科学家科研奖励基金;山东省教育厅科学技术项目

The Effect of Culture Time on Activity of Cercospora zeae-maydis Toxins

Wang Guiqing1,2, Zhang Tao2, Sun Hua2, Xu Xiude1, Dong Huaiyu1   

  • Received:2009-12-21 Revised:2010-01-14 Online:2010-05-05 Published:2010-05-05

PDF (PC)

951

摘要：

通过抑制胚根伸长法测定了培养时间对玉米灰斑病菌毒素产量和活性的影响，结果表明，以Fries培养液为基质，静止培养15d时，毒素的产量最高，为38.477mg/mL，毒素的活性最强，对玉米胚根生长的抑制中浓度为18.012%。说明Fries培养液中静止培养15d最适合玉米灰斑病菌毒素的产生。该研究为进一步优化玉米灰斑病菌的产毒条件奠定了基础。

关键词: 蛋用鹌鹑, 蛋用鹌鹑, 体温, 昼夜, 相对湿度, 规律性

Abstract:

The effects of culture time on production and activity of Cercospora zeae-maydis toxins have been studied in this article. The mensuration of inhibition of radicle growth was used to examine the activity of the toxins. The results showed that the maximum production and the strongest inhibitory activity of Cercospora zeae-maydis toxins were obtained when it was statically cultured in the Fries nutrient fluid for 15ds. The maximum production of the toxins is 38.477mg/mL. The median inhibitory concentration on maize radicle growth of the toxins is 18.012%. This indicated that the statically cultured in the Fries nutrient fluid for 15ds was the most fitting formation conditions of Cercospora zeae-maydis toxins. The results of this study will provide the foundation for optimization of formation conditions of Cercospora zeae-maydis toxins.