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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 47-52.

• 生物技术科学 • 上一篇    下一篇

肇东苜蓿和Pleven6苜蓿离体再生体系的建立

麻晓春 张月学 张海玲 尚晨 李佶恺 徐香玲   

  • 收稿日期:2009-12-23 修回日期:2010-03-12 出版日期:2010-07-05 发布日期:2010-07-05

Alfalfa Establishment of in Vitro Regeneration System of Zhaodong Alfalfa and Pleven6

Ma Xiaochun1, Zhang Yuexue2, Zhang Hailing2, Shang Chen2, Li Jikai2, Xu Xianglin   

  • Received:2009-12-23 Revised:2010-03-12 Online:2010-07-05 Published:2010-07-05

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摘要：

以肇东紫花苜蓿和Pleven6紫花苜蓿的子叶、下胚轴为外植体，建立离体再生体系。结果如下：最佳愈伤诱导培养基：肇东苜蓿为MS+2.0mg/L2,4-D+0.5mg/ＬKT+3%蔗糖，Pleven6为MS+1.5mg/L2,4-D+0.5mg/LKT+3%蔗糖；分化培养基：MS+2.0mg/L6-BA+0.5mg/L NAA+3%蔗糖；分化继代培养基：MS+0.3mg/L6-BA+0.1mg/LNAA+0.3mg/LKT+3%蔗糖；生根培养基：1/2MS+0.5mg/LNAA+1.5%蔗糖。两个品种的子叶均较下胚轴分化时间短、分化效率高，肇东苜蓿诱导率和分化率明显高于pleven6苜蓿。

关键词: K型胞质, K型胞质, 不育系, 高异交力, 发掘, 利用研究

Abstract:

The cotyledons and hypocotyls explants from Zhaodong alfalfa and Pleven6 alfalfa ，the establishment of in vitro regeneration system. The results are as follows : the best callus induction medium of Zhaodong alfalfa was MS +2 .0mg/L2,4-D +0.5 mg / LKT +3% sucrose and of Pleven6 was MS +1.5 mg/L2,4-D +0.5 mg / LKT +3% sucrose; Differentiation medium: MS +2.0 mg/L6-BA +0.5 mg / LNAA +3% sucrose; Differentiation subculture medium: MS +0.3 mg/L6-BA +0.1 mg / LNAA +0.3 mg / LKT +3% sucrose; Induced rooting medium: 1/2MS +0.5 mg / LNAA +1.5% sucrose. At the same level of the experiment, induction rate and differentiation rate of Zhaodong alfalfa was significantly higher than pleven6 alfalfa, and both cotyledons used shorter differentiation time and higher differentiation rate than hypocotyls.