欢迎访问《中国农学通报》,

中国农学通报 ›› 2022, Vol. 38 ›› Issue (18): 133-139.doi: 10.11924/j.issn.1000-6850.casb2021-1221

所属专题: 生物技术 水稻 农业生态

• 植物保护·农药 • 上一篇    下一篇

分子标记辅助选择改良水稻不育系‘荃211S’白叶枯病的抗性

黄艳玲(), 严志, 申广勒, 王慧, 张从合(), 杨韦, 陈琳, 张云虎, 庞战士, 乔木, 李方宝, 杨力   

  1. 安徽荃银高科种业股份有限公司/农业农村部杂交稻新品种创制重点实验室,合肥 230088
  • 收稿日期:2021-11-20 修回日期:2022-03-28 出版日期:2022-06-25 发布日期:2022-07-14
  • 通讯作者: 张从合
  • 作者简介:黄艳玲,女,1979年出生,安徽固镇人,高级农艺师,研究生,硕士,主要从事水稻遗传育种工作。通信地址:230088 安徽省合肥市创新大道98号 安徽荃银高科种业股份有限公司,Tel:13856012765,E-mail: 56018238@qq.com
  • 基金资助:
    安徽省科技重大专项“优质、多抗、高产杂交水稻种质资源及品种精准高效培育及应用”(201903a06020011)

Bacterial Blight Resistance of Rice Sterile Line ‘Quan 211S’ Improved by Molecular Marker-Assisted Selection

HUANG Yanling(), YAN Zhi, SHEN Guangle, WANG Hui, ZHANG Conghe(), YANG Wei, CHEN Lin, ZHANG Yunhu, PANG Zhanshi, QIAO Mu, LI Fangbao, YANG Li   

  1. Anhui, Win-All Hi-Tech Seed Co., Ltd.,/ Key Lab for New Variety Development of Hybrid Rice of Ministry of Agriculture and Rural Affairs, Hefei 230088
  • Received:2021-11-20 Revised:2022-03-28 Online:2022-06-25 Published:2022-07-14
  • Contact: ZHANG Conghe

摘要:

本研究旨在提高优质两系光温敏型核不育系‘荃211S’的白叶枯病抗性。以‘荃211S’为受体亲本,以含有抗白叶枯病Xa23基因的CBB23为供体亲本,通过分子标记辅助选择技术与常规育种技术相结合,筛选抗白叶病的不育株系。筛选出4个改良不育株系QK211S-1~QK211S-4。改良‘荃211S’株系的农艺性状、育性转换温度、异交结实率与‘荃211S’相近,但在白叶枯病抗性上明显增强,表现出高抗或抗,抗性级别0~2。改良‘荃211S’株系所配组合与‘荃211S’所配组合在株高、每穗总粒数、结实率、千粒重的差异不显著,但白叶枯病抗性显著增强,抗性级别为1,高抗。改良的4个‘荃211S’株系为选育抗病水稻品种奠定了基础,可以直接在生产中应用。

关键词: 水稻, 分子标记辅助选择, 白叶枯病, Xa23基因, 两系不育系

Abstract:

This study aims to improve the bacterial blight resistance of photo-thermo-sensitive genic male sterile ‘Quan 211S’ with high quality. Using ‘Quan 211S’ as the recipient parent and ‘CBB23’ containing Xa23 gene as the donor parent, the sterile lines resistant to bacterial blight were bred by combining molecular marker-assisted selection technology with conventional breeding technology. Four improved sterile lines, from Qk211s-1 to Qk211s-4 were selected. The agronomic characters, fertility alteration temperature and outcrossing setting rate of the improved lines of ‘Quan 211S’ were similar to those of ‘Quan 211S’, but the resistance to bacterial blight was significantly enhanced, showing high resistance or resistance, and the resistance level was 0-2. The combinations of improved lines of ‘Quan 211S’ had no significant differences in plant height, grain number per panicle, seed setting rate and 1000-grain weight compared with those of the combinations of ‘Quan 211S’, but the resistance to bacterial blight was significantly enhanced, showing high resistance, and the resistance level was 1. The four improved lines of ‘Quan 211S’ can lay a foundation for breeding disease resistant rice varieties and be directly applied in production.

Key words: rice, molecular marker-assisted selection, bacterial blight, Xa23 gene, dual-purpose genic sterile line

中图分类号: