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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 255-258.

• 林学 园艺 园林 • 上一篇    下一篇

不同激素对黄檗腋芽丛生芽苗诱导及种质试管保存的影响

顾地周1,高捍东2,冯颖1,姜云天1   

  • 收稿日期:2009-12-28 修回日期:2010-01-08 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    长白山重要珍稀药用植物仿生栽培及保护研究

The effect of various hormones to induce multiple shoot clumps from axillary bud of Phellodendron amurense and germplasm peservation in vitro

Gu Dizhou1, Gao Handong2, Feng Ying1, Jiang Yuntian1   

  • Received:2009-12-28 Revised:2010-01-08 Online:2010-05-05 Published:2010-05-05

PDF (PC)

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摘要：

以黄檗嫩茎为外植体，研究不同激素对黄檗嫩茎腋芽丛生芽苗、芽苗生根和试管苗保存的影响。应用均匀设计法筛选其离体培养和种质试管保存各阶段最适合的培养基。结果表明，最适合诱导嫩茎腋芽丛生芽苗的培养基为：N6+TDZ2.86 mg?L-1 +IAA 0.03 mg?L-1，诱导率为99.5%；生根培养基为：1/2N6+KT 0.45 mg?L-1+IBA 0.10 mg?L-1，生根率达97%以上；试管苗保存培养基为：N6 +根皮苷2. 65 mg?L-1 +KT 0.15 mg?L-1，在常温条件下，在试管内保存黄檗种质可达45个月以上，生长率仅达0.74%。通过腋芽丛生和延缓生长的方法，成功建立了黄檗的离体培养和种质试管保存体系。

关键词: 苜蓿, 苜蓿, BADH基因, 抗性

Abstract:

The tender stems of Phellodendron amurense Rupr. was used as explant, studied the effect of various hormones to induce multiple shoot clumps from axillary bud of Phellodendron amurense, root of shoots and germplasm peservation in vitro. Uniform design experiments for screened the suitable medium compositions of in vitro cultured and germplasm peservation in vitro. The results showed that N6+TDZ2.86 mg?L-1 +IAA 0.03 mg?L-1 for axillary bud multiple shoot clumps from tender stem, the rate of induction was 99.5%; 1/2N6+KT 0.45 mg?L-1+IBA 0.10 mg?L-1 for rooting, the rate of rooting was more than 97%; N6+phlorizin2. 65 mg?L-1+KT 0.15 mg?L-1 for germplasm preservation in vitro for 45 months by the retarding of growing at normal temperature, the rate of growth was 0.74%. In vitro culture and germplasm conservation in vitro system of Phellodendron amurense have been successfully established by the methods of inducing axillary bud multiple shoot clumps and the retarding of growing.