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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (12): 221-226.

所属专题： 生物技术； 园艺

• 植物保护 农药 • 上一篇    下一篇

橡胶树炭疽病菌的鉴定及rDNA-ITS序列分析

李继锋1,2,刘先宝2,蔡吉苗2,林春花2,黄贵修2   

  • 收稿日期:2010-01-04 修回日期:2010-01-12 出版日期:2010-06-20 发布日期:2010-06-20
  • 基金资助:

    热带特色经济作物重大病虫害防控技术研究与示范项目资助

Identification and the internal transcribed spacer regions analysis of pathogen causing rubber anthracnose

Li Jifeng1,2, LiuXianbao2, Cai Jimiao2, Lin Chunhua2, Huang Guixiu2   

  • Received:2010-01-04 Revised:2010-01-12 Online:2010-06-20 Published:2010-06-20

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摘要：

摘要：从我国海南和云南橡胶主产区分离到22株橡胶炭疽病菌，生物学特性和形态学观察表明其中存在尖孢炭疽菌，ITS序列分析进一步证明侵染我国海南和云南橡胶的炭疽菌为胶孢炭疽和尖孢炭疽。ITS序列多样性分析表明，15株尖孢炭疽菌之间遗传差异较小，7株胶孢炭疽菌之间差异较大。

关键词: 水肥交互, 水肥交互, 南瓜, 光合特性, 产量

Abstract:

Abstract: The internal transcribed spacer regions (5.8S/ITS) of the ribosomal RNA gene from 22 isolates of colletotrichum leaf disease of rubber were amplified through the polymerase chain reaction with the universal primers and sequenced. And their biological characters also tested. The result revealed that only 7 isolates belong to Colletotrichum gloeosporioides. The remaining 15 isolates represented Colletotrichum acutatum. Intraspecific genetic variation of Colletotrichum acutatum isolated from rubber was relatively low, which was found through rDNA-ITS sequence analyses. However, intraspecific genetic variation of Colletotrichum gloeosporioides was relatively abundant.