欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 395-401.

• 水产 渔业 • 上一篇    下一篇

牙鲆亲本间遗传距离与其后代生长速度的相关性分析

毕金贞 陈松林   

  • 收稿日期:2010-03-09 修回日期:2010-04-29 出版日期:2010-08-05 发布日期:2010-08-05
  • 基金资助:

    国家科技支撑计划;“863”高技术研究发展计划

Correlation between Genetic Distances among Parents and Growth Rate of Offspring in Paralichthys olivaceus

  • Received:2010-03-09 Revised:2010-04-29 Online:2010-08-05 Published:2010-08-05

PDF (PC)

1038

摘要：

分子标记技术为直接从DNA水平上认识生物个体遗传差异提供了强有力的工具，用其分析亲本间遗传距离与后代生长性状之间的关系是水产育种研究的重要方向之一。选用21个多态性微卫星分子标记，以8个日本牙鲆（JS）个体和24个抗病牙鲆（RS）个体为亲本产生的28个杂交组合为材料，分析了该32个牙鲆亲本的遗传多样性，同时对牙鲆亲本间遗传距离与其后代生长速度的相关性进行了研究。结果表明：（1）21个微卫星标记共扩增出123个等位基因，32个牙鲆亲本中的等位基因数(A)，有效等位基因数(Ae)，观测杂合度(Ho)，期望杂合度(He)，多态信息含量（PIC）分别在2~15，1.3423~7.8168，0.2333~1.000，0.2593~0.8859，0.2225~0.8613之间变动，相应的平均值分别为5.86，3.7951，0.6931，0.6784，0.6237。（2）进行亲本间遗传距离（GD）与其后代生长速度（AGRw）的相关性分析发现，亲本间最小遗传距离为0.2578，最大遗传距离为0.9773，不同遗传距离范围内，亲本间遗传距离与后代生长速度之间呈现不同的相关性：在0.2578~0.5958范围内，亲本间遗传距离与后代生长速度呈显著的正相关（P<0.05）；在0.6099~0.6604范围内，亲本间遗传距离与后代生长速度之间没有表现出显著的相关性；在0.6640~0.9773范围内，亲本间遗传距离与后代生长速度呈显著的负相关（P<0.05）。

关键词: 甜瓜, 甜瓜, 白粉病, 抗性, 防御酶活性

Abstract:

Molecular marker technology provides a powerful tool for understanding the genetic differences between individuals from the DNA level directly.It is an important direction to analyse the correlation between parental genetic distance and the growth traits of their offspring with the DNA molecular markers. Eight Japanese flounders(JS) and 24 resistant flounders(RS) were used to make 28 crosses to analyse the genetic diversity of 32 parents,using 21 polymorphic microsatellite markers.Simultaneously, the correlation between parent SSR genetic distance and the growth rate of their offspring was assayed.The results indicated that:(1) the number of total alleles of these 21 microsatellite loci was 123，the allele number (A)，effective allele number (Ae)，observed heterozygosity (Ho)，expected heterozygosity (He) and polymorphism information content（PIC），ranged from 2~15，1.3423~7.8168，0.2333~1.000，0.2593~0.8859，0.2225~0.8613，respectively.The average A，Ae，Ho，He，PIC was 5.86, 3.7951,0.6931,0.6784,0.6237，respectively. (2)The minimum genetic distance was 0.2578,while the maximum was 0.9773.The correlation between AGRw of offspring and parental GD existed considerable difference in different range of genetic distance. As the GD for 0.2578~0.5958, the correlation between AGRw of offspring and parental GD appeared to be positively significant at P<0.05；As the GD for 0.6099~0.6604，there was no significant correlation；Furthermore,as the GD for 0.6640~0.9773,the correlation between AGRw of offspring and parental GD appeared to be negatively significant at P<0.05.