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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 57-60.

• 生物技术科学 • 上一篇    下一篇

茯苓栽培菌株的RAPD分析

蔡丹凤 陈美元 郭仲杰 李洪荣 王雪英 王泽生   

  • 收稿日期:2010-03-10 修回日期:2010-04-21 出版日期:2010-10-20 发布日期:2010-10-20
  • 基金资助:

    福建省科技厅重点项目

RAPD Analysis of Cultivation Strains of Wolfiporia cocos

  • Received:2010-03-10 Revised:2010-04-21 Online:2010-10-20 Published:2010-10-20

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摘要：

笔者应用RAPD分子标记技术对14个茯苓栽培菌株进行遗传多样性分析，以期探究茯苓栽培菌株间的亲缘关系，为茯苓菌株的鉴别及育种提供DNA依据。研究中应用9个RAPD引物对茯苓栽培菌株进行RAPD分析，筛选出4个RAPD引物，扩增出21条比较明显的条带。利用21条扩增带对14个菌株进行聚类分析，获得14个茯苓栽培菌株亲缘关系树状图。结果显示在53%的相似值时，14个茯苓栽培菌株可分为2大类群，而在93%相似值时可分为13个类群。

关键词: 龙眼, 龙眼, 胚性愈伤组织, GPX, NaCl, 光, 温度

Abstract:

In this paper, the genetic diversity of 14 cultivation strains of Wolfiporia cocos were analyzed by using the molecular labeling technique of RAPD to study their genetic relationship. 9 RAPD random primers were used in the amplification and 4 of them produced 21 relative obvious DNA bands. The strains were clustered base on the 21 DNA bands and a dendrogram of 14 W.cocos cultivation strains was obtained. The results showed that 14 W.cocos strains could be divided into 2 groups at the similarity coefficient of 53% ;while at the similarity coefficient of 93%, the strains were divided into 13 groups.