脱落酸对葡萄幼苗镉积累的影响

doi:10.11924/j.issn.1000-6850.casb2021-0764

中国农学通报 ›› 2022, Vol. 38 ›› Issue (34): 46-51.doi: 10.11924/j.issn.1000-6850.casb2021-0764

所属专题：生物技术；园艺

脱落酸对葡萄幼苗镉积累的影响

韩佳希¹^,²(), 范中菡¹, 董义霞³, 吕昕芮², 李红春⁴, 陈庆华¹, 李洪浩¹, 林立金², 胡容平⁵()

¹四川省农业科学院植物保护研究所/农业部西南作物有害生物综合治理重点实验室，成都 610066
²四川农业大学园艺学院，成都 611130
³凉山彝族自治州植物检疫站，四川西昌 615050
⁴眉山市彭山区农业农村局，四川眉山 620860
⁵四川省食用菌研究所，成都 610066

收稿日期:2021-08-10 修回日期:2022-07-26 出版日期:2022-12-05 发布日期:2022-11-25
通讯作者: 胡容平
作者简介:韩佳希，女，2001年出生，四川乐山人，本科生，主要从事园艺植物栽培生理研究。通信地址：611130 四川省成都市温江区公平街道惠民路211号四川农业大学园艺学院，E-mail：1263551401@qq.com。
基金资助:
国家现代农业产业体系四川水果创新团队项目“葡萄、猕猴桃病虫害绿色防控”(sccxtd-2022-04);四川省农业科学院中试熟化项目“丹棱县特色水果提质增效绿色生产技术示范”(2021ZSSFXC56)

Effect of Abscisic Acid on Cadmium Accumulation of Grape Seedlings

HAN Jiaxi¹^,²(), FAN Zhonghan¹, DONG Yixia³, LV Xinrui², LI Hongchun⁴, CHEN Qinghua¹, LI Honghao¹, LIN Lijin², HU Rongping⁵()

¹Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/ Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops of Ministry of Agriculture and Rural Affairs, Chengdu 610066
²College of Horticulture, Sichuan Agricultural University, Chengdu 611130
³Plant Quarantine Station of Liangshan Yi Autonomous Prefecture, Xichang, Sichuan 615050
⁴Agricultural and Rural Bureau of Pengshan District, Meishan, Sichuan 620860
⁵Institute of Sichuan Edible Fungi, Chengdu 610066

Received:2021-08-10 Revised:2022-07-26 Online:2022-12-05 Published:2022-11-25
Contact: HU Rongping

摘要/Abstract

摘要：

为降低镉污染条件下葡萄对镉的积累，促进葡萄生长，采用盆栽试验研究镉污染(5 mg/kg)条件下喷施脱落酸对葡萄幼苗生长及镉积累的影响。结果表明，葡萄幼苗各部位的生物量和叶片的光合色素含量均随着脱落酸浓度的增加(0、1、5、10、20 μmol/L)而先降低后增加。随着脱落酸浓度的增加，葡萄幼苗叶片的POD及CAT活性和土壤pH呈现先增加后降低的趋势，而SOD活性随着脱落酸浓度的增加而增加。另外，喷施脱落酸能使葡萄幼苗各部位镉含量显著低于对照，并随着脱落酸浓度的增加而减少，脱落酸浓度为20 μmol/L时，葡萄幼苗的根系和地上部分分别较对照降低了36.54%和74.36%。综上，喷施脱落酸能够降低葡萄对镉的吸收，以20 μmol/L脱落酸浓度最佳。

关键词: 葡萄, 镉, 脱落酸, 光合生理, 抗氧化生理

Abstract:

To reduce the cadmium accumulation in grape and promote the grape growth under the cadmium pollution condition, a pot experiment was used to study the effect of spraying abscisic acid on the growth and cadmium accumulation of grape seedlings under cadmium pollution (5 mg/kg). The results showed that the biomass of each part of the grape seedlings and the photosynthetic pigment content in leaves all decreased first and then increased with the increase of the spraying concentration of abscisic acid (0, 1, 5, 10, and 20 μmol/L). Compared with those of control (0 μmol/L), with the increase of abscisic acid concentration, the POD and CAT activities of grape seedling leaves and the soil pH value increased first and then decreased, while SOD activity increased with the increase of abscisic acid concentration. In addition, spraying abscisic acid could make the cadmium content of each part of grape seedlings significantly lower than that of the control, and decrease with the increase of abscisic acid concentration. When the concentration of spraying abscisic acid was 20 μmol/L, the cadmium content of the roots and above-ground parts of grape seedlings decreased by 36.54% and 74.36% respectively compared with that of control. In summary, spraying abscisic acid could inhibit the cadmium absorption of grape, and the abscisic acid concentration of 20 μmol/L is the best.

Key words: grape, cadmium, abscisic acid, photosynthetic physiology, antioxidant physiology

中图分类号:

X53，S663.1，Q946.885+.6

韩佳希, 范中菡, 董义霞, 吕昕芮, 李红春, 陈庆华, 李洪浩, 林立金, 胡容平. 脱落酸对葡萄幼苗镉积累的影响[J]. 中国农学通报, 2022, 38(34): 46-51.

HAN Jiaxi, FAN Zhonghan, DONG Yixia, LV Xinrui, LI Hongchun, CHEN Qinghua, LI Honghao, LIN Lijin, HU Rongping. Effect of Abscisic Acid on Cadmium Accumulation of Grape Seedlings[J]. Chinese Agricultural Science Bulletin, 2022, 38(34): 46-51.

图/表 6

参考文献 41

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脱落酸浓度	根系	茎杆	叶片	地上部分
0 μmol/L	1.546±0.035bc	1.121±0.007b	2.628±0.051ab	3.749±0.058ab
1 μmol/L	1.186±0.013d	0.639±0.008e	2.181±0.073c	2.819±0.080d
5 μmol/L	1.425±0.039c	0.699±0.012d	2.514±0.070b	3.213±0.081c
10 μmol/L	1.532±0.032b	0.909±0.018c	2.620±0.071ab	3.529±0.089b
20 μmol/L	1.664±0.036a	1.258±0.025a	2.746±0.061a	4.004±0.086a

脱落酸浓度	根系	茎杆	叶片	地上部分
0 μmol/L	1.546±0.035bc	1.121±0.007b	2.628±0.051ab	3.749±0.058ab
1 μmol/L	1.186±0.013d	0.639±0.008e	2.181±0.073c	2.819±0.080d
5 μmol/L	1.425±0.039c	0.699±0.012d	2.514±0.070b	3.213±0.081c
10 μmol/L	1.532±0.032b	0.909±0.018c	2.620±0.071ab	3.529±0.089b
20 μmol/L	1.664±0.036a	1.258±0.025a	2.746±0.061a	4.004±0.086a

脱落酸浓度	叶绿素a	叶绿素b	叶绿素总量	类胡萝卜素
0 μmol/L	1.031±0.025b	0.515±0.012ab	1.546±0.036b	0.197±0.007a
1 μmol/L	0.663±0.016c	0.455±0.009c	1.119±0.026c	0.138±0.006b
5 μmol/L	0.987±0.018b	0.469±0.008c	1.457±0.026b	0.153±0.003b
10 μmol/L	1.000±0.040b	0.481±0.013bc	1.481±0.053b	0.156±0.004b
20 μmol/L	1.159±0.028a	0.545±0.015a	1.704±0.043a	0.209±0.008a

脱落酸浓度	叶绿素a	叶绿素b	叶绿素总量	类胡萝卜素
0 μmol/L	1.031±0.025b	0.515±0.012ab	1.546±0.036b	0.197±0.007a
1 μmol/L	0.663±0.016c	0.455±0.009c	1.119±0.026c	0.138±0.006b
5 μmol/L	0.987±0.018b	0.469±0.008c	1.457±0.026b	0.153±0.003b
10 μmol/L	1.000±0.040b	0.481±0.013bc	1.481±0.053b	0.156±0.004b
20 μmol/L	1.159±0.028a	0.545±0.015a	1.704±0.043a	0.209±0.008a

脱落酸/(μmol/L)	POD活性/[U/(g·min)]	SOD活性/(U/g)	CAT活性/(mg/g)
0	15.63±0.32b	102.41±2.63c	0.761±0.012c
1	17.71±0.50a	157.72±4.62b	1.110±0.037b
5	18.15±0.45a	173.43±0.52a	1.352±0.032a
10	14.95±0.21b	177.79±5.18a	1.262±0.044a
20	13.68±0.14c	183.95±5.37a	0.815±0.024c

脱落酸对葡萄幼苗镉积累的影响

Effect of Abscisic Acid on Cadmium Accumulation of Grape Seedlings

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脱落酸浓度	根系	茎杆	叶片	地上部分
0 μmol/L	32.59±0.41a	1.341±0.045a	1.041±0.031a	1.131±0.007a
1 μmol/L	24.79±0.13b	0.639±0.018b	0.296±0.006b	0.374±0.010b
5 μmol/L	23.88±0.32bc	0.588±0.007c	0.297±0.004b	0.360±0.002bc
10 μmol/L	22.57±0.56c	0.498±0.008d	0.294±0.006b	0.346±0.006c
20 μmol/L	20.68±0.54d	0.437±0.011d	0.223±0.007c	0.290±0.001d

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