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中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 422-426.

所属专题: 水产渔业

• 水产 渔业 • 上一篇    下一篇

实时荧光定量PCR技术及其在水产上的应用

唐永凯 俞菊华 徐跑 李建林 李红霞 胡海彦 阮瑞霞   

  • 收稿日期:2010-03-26 修回日期:2010-06-10 出版日期:2010-11-05 发布日期:2010-11-05

Real-time Fluorescent Quantitative PCR Technique and its Application in Aquaculture

  • Received:2010-03-26 Revised:2010-06-10 Online:2010-11-05 Published:2010-11-05

摘要:

摘要:实时荧光定量PCR技术是在PCR技术基础上结合荧光染料发展起来的一种核酸定量技术,能快速、灵敏,并有效地对核酸进行定量检测,已被广泛应用于生命科学的各领域。本文就实时荧光定量PCR的检测原理、荧光染料,管家基因以及其在水产上的应用作一阐述。

关键词: 土地整理, 土地整理, 土壤肥力保持价值, 整理类型

Abstract:

Abstract: The technology of real-time fluorescent quantification PCR stems from the technology of PCR and fluorescent dye. It has developed a method of calculating nuclear amount and has been widely used in all fields of life science. Here we elucidate the principle of detecting, fluorescent dye, house-keeping gene and the application in aquaculture about real-time fluorescent quantification PCR.