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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 279-283.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

放线菌菌株97发酵液中活性代谢产物的抑菌作用及稳定性测定

张茹萍 郑洪波 贾春燕 贾玉 郑翠梅 张广民   

  • 收稿日期:2010-04-06 修回日期:2010-05-25 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    山东省烟草专卖局科技项目

Study on Antimicrobial Activity and Stability of Bioactive Metabolite Produced by Actinomycete 97 Strain Fermentation

  • Received:2010-04-06 Revised:2010-05-25 Online:2011-09-20 Published:2011-09-20

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摘要： 本实验室从采自山东临沂和日照的烟田土样中分离得到一株对烟草黑胫病菌(Phytophthora parasitica var. nicotianae)具有拮抗作用的放线菌菌株97，为了明确该生防菌的抑菌作用和抑菌因子的理化性质，采用菌丝生长速率法，对菌株97发酵液中活性代谢产物的抑菌作用及稳定性进行了研究。结果表明，发酵液中活性代谢产物对多种植物病原真菌均表现较好的抑制作用。此外，发酵液中活性代谢产物在低温贮藏下仍有很好的抑菌效果，在偏酸性或中性条件下稳定，有较强的热稳定性和抗紫外线照射能力，对蛋白酶K稳定性较好。

关键词: 条华蜗牛, 条华蜗牛, 翻身时间, 体重, 温度, 光照, 饥饿, 取食

Abstract: Strain 97of Actinomycetes with high activity to P. parasitica var. nicotianae was isolated from the tobacco field soil sampls collected in Linyi and Rizhao areas of Shandong province. A study on antifungal action and stability of bioactive metabolite produced by Actinomycete strain 97 fermentation was launched by means of growth speed method for the sake of evaluating the antifungal action and physicochemical characteristics of inhibitive factors of strain 97.The results showed that the bioactive metabolite had a broad antifungal spectrum and exhibited antagonistic abilities to several plant pathogens.In addition,it still had a high activity when stored up at 4℃,and was stable to acidic,neutral and heat,maintained the antifungal activity after treatment of proteinase K and ultraviolet radiation.