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中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 194-199.

所属专题: 生物技术 园艺

• 农学 农业基础科学 • 上一篇    下一篇

己糖激酶调控赤霞珠酿酒葡萄蔗糖分解代谢

杨培培 王秀芹 黄卫东 战吉宬   

  • 收稿日期:2010-04-23 修回日期:2010-05-12 出版日期:2010-07-05 发布日期:2010-07-05
  • 基金资助:

    国家自然基金

Hexokinase regulate sucrose cleavage catabolism during the development of Cabernet Sauvignon

  • Received:2010-04-23 Revised:2010-05-12 Online:2010-07-05 Published:2010-07-05

摘要:

摘 要:以酿酒葡萄(Vitis vinifera L.)赤霞珠果实为试材,研究己糖激酶与蔗糖分解代谢关键酶[可溶性酸性转化酶和蔗糖合酶(分解方向)]在果实发育过程中的关系。利用高效液相色谱(HPLC)分析了发育期赤霞珠葡萄果实中蔗糖、葡萄糖和果糖的含量,测定了其己糖激酶、酸性转化酶和蔗糖合酶活性。研究表明果实整个发育过程中,蔗糖含量极微,己糖激酶表现出与葡萄糖、果糖相反的梯度变化;葡萄糖和果糖含量低时,可溶性酸性转化酶和蔗糖合酶的活性开始上升;葡萄糖和果糖含量高时,可溶性酸性转化酶和蔗糖合酶的活性降低。相关性分析表明,蔗糖含量与葡萄糖含量、果糖含量显著相关,葡萄糖和果糖含量与可溶性酸性转化酶、蔗糖合酶活性负相关,己糖激酶活性与所有因子负相关,但无显著相关。

关键词: 缓慢降温, 缓慢降温, 石榴, 冷害

Abstract:

Abstract: Wine grape(Vitis vinifera L.)Cabernet Sauvignon fruit were used to study the relationship of Hexokinase and key sucrose metabolism [enzymes acid invertase and sucrose synthase activity(cleavage direction)]. Using HPLC analyse the content of sucrose, glucose and fructose in berry; and the hexokinase, acid invertase and sucrose synthase enzyme activity are checked . During the development, the sucrose content was small; The gradient of Hexokinase compared with glucose, fructose was opposite; When glucose and fructose content was low, the soluble acid invertase and sucrose synthase activity began to rise; When glucose and fructose content was high, the soluble acid invertase and sucrose synthase decreased.Correlation analysis showed that sucrose content was significantly related to glucose and fructose content, glucose and fructose content was significantly negatively related with soluble acid invertase and sucrose synthase activity. hexokinase activity negatively correlated with all factors, but no significant correlation.