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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 43-47.

• 生物技术科学 • 上一篇    下一篇

半夏遗传结构和多样性RAPD标记分析

张君毅   

  • 收稿日期:2010-06-29 修回日期:2010-07-15 出版日期:2011-09-20 发布日期:2011-09-20
  • 基金资助:
    福建省自然科学基金项目;华侨大学人才引进项目

Genetic Structure and Diversity among Pinellia ternata Revealed by RAPD Markers

  • Received:2010-06-29 Revised:2010-07-15 Online:2011-09-20 Published:2011-09-20

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摘要： 利用RAPD标记技术对取自半夏主要分布区16个省的24份种质进行遗传多样性分析。结果表明：在物种水平上，多态位点百分率（PPL）68.77%，Nei’S基因多样性指数（He）0.2062；居群水平上，PPL为9.88%～58.02%，平均27.21%，群体间遗传分化系数（GST）0.5282，居群间基因流（Nm）0.4466。UPGMA聚类分析显示，当相似系数0.896时，24份种质可分为5个类群。

关键词: 福美双, 福美双, 戊唑醇, 小麦赤霉病, 复配, 细胞膜透性, 内含物

Abstract: Random amplification of polymorphic DNA (RAPD) marker was occupied to study on the genetic diversity of 24 Pinellia ternata germplasm resources from16 main distribution provinces. The results showed .that at species level polymorphic loci percentage (PPL) is 68.77%, Nei 'S gene diversity index (He) 0.2062. At population level, PPL varied from 9.88% to 58.02%, with the average value 27.21% and groups of genetic differentiation factor (GST) is 0.5282, population gene flow (Nm) 0.4466. The results of cluster analysis based on UPGMA revealed that when similar coefficient of 0.896, 24 populations can be divided into five material groups.