欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (18): 247-250.

• 林学 园艺 园林 • 上一篇    下一篇

千层金组培快繁技术研究

吴维坚 林加根 鞠玉栋 杨敏   

  • 收稿日期:2010-08-03 修回日期:2010-08-26 出版日期:2011-09-20 发布日期:2011-09-20

Study on Tissue Culture and Rapid Propagation Technology for Melaleuca bracteata

  • Received:2010-08-03 Revised:2010-08-26 Online:2011-09-20 Published:2011-09-20

PDF (PC)

999

摘要： 以千层金茎段为外植体进行离体培养，通过试验筛选出各阶段适宜的培养基，建立了千层金组培快繁体系。结果表明：在诱导不定芽的培养基中，MS+6-BA 1 mg/L的芽萌发率最高，芽体健壮；丛生芽继代增殖培养基为改良MS+6-BA 1 mg/L+NAA 0.1 mg/L+KT 0.1 mg/L；生根培养基为1/2MS+1号生根剂0.5 mg/L+IBA 0.1 mg/L，生根率达100%，且根系发达；栽培基质以椰糠+腐殖土=2:1较理想，移栽成活率为89.3%。

关键词: 展望, 展望

Abstract: The stem segments of Melaleuca bracteata was used as explants, the tissue culture and rapid propagation technology system was established. The results showed that the best initiated medium was MS+6-BA 1 mg/L, and the best proliferation medium was MS+6-BA 1 mg/L+NAA 0.1 mg/L+KT 0.1 mg/L. The optimum shoots rooting medium was 1/2MS＋IBA 0.1 mg/L, with the rooting of 100% and developed roots. The survival rate of tissue culture plantlets was over 89.3% after the plantlets acclimation transplanting experiment.