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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (4): 76-80.

• 林学 园艺 园林 • 上一篇    下一篇

枣疯病植原体的快速检测

余贤美 单公华 吕菲菲 沈广宁 周广芳   

  • 收稿日期:2010-09-03 修回日期:2010-11-03 出版日期:2011-02-10 发布日期:2011-02-10
  • 基金资助:

    主要果树新品种选育与高标准生产技术研究

Rapid Determination of Jujube Witches’ Broom Phytoplasma

  • Received:2010-09-03 Revised:2010-11-03 Online:2011-02-10 Published:2011-02-10

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摘要：

以表现丛枝症状的‘金丝4号’枣树为试材，以叶片总DNA粗提物为模板通过PCR扩增技术克隆16S rDNA基因，建立了枣疯病植原体快速检测方法。序列分析结果显示，枣疯病植原体‘金丝4号’株系（JWB-Jinsi4，TA）与JWB-G1（AB052876）同源性为99.7%，归属于16Sr Ⅴ-B组。实验结果表明，以DNA粗提物为模板的PCR扩增技术，可快速有效地检测枣疯病植原体，为生产实践中枣疯病的诊断和防治提供技术支持。

关键词: 二次正交旋转设计, 二次正交旋转设计, 刺五加, 组织培养

Abstract:

A rapid determination method was established by 16S rDNA sequence analysis via PCR amplification from the crude DNA extract of ‘Jinsi4’ jujube leaves with witches’ symptoms. The results showed that Jujube witches’ broom phytoplasma strain JWB-Jinsi4,TA shared the identity of 99.7% with Jujube witches’ broom phytoplasma strain JWB-G1（AB052876）and was classified as a member of 16Sr Ⅴ-B group, which indicated that PCR amplification method using crude DNA extract as the template could be used to detect Jujube witches’ broom phytoplasma, and provide the technique support for the diagnosis and control of jujube witches’ broom in productive practice.

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