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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 342-349.

所属专题： 生物技术

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

纤维素热解产物内醚糖的微生物同化与乙醇发酵测试

余志晟 宁洎英 张 玲 张洪勋   

  • 收稿日期:2010-09-29 修回日期:2010-12-01 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    酵母菌内醚糖激酶功能基因的克隆与表达;北京市科技新星项目

Microbial Investigation on Assimilation and Ethanol Fermentation of Levoglucosan from Cellulosic Pyrolysis Products

  • Received:2010-09-29 Revised:2010-12-01 Online:2011-03-20 Published:2011-03-20

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摘要：

以纤维素的热解产物1,6-缩水-β-D-吡喃葡萄糖（Levoglucosan，简称内醚糖）为唯一碳源对89株微生物（大部分为产酒菌株）进行了同化和乙醇发酵测试，并对319份土样进行了分离纯化培养。筛选结果表明，在89株微生物中，一株Sporobolomyces酵母、一株Rhodotorula酵母、4株Rhizopus霉菌、2株Monascus霉菌有同化内醚糖的能力，但它们利用内醚糖的能力都较弱，在培养3天后，2%内醚糖的利用率低于30%；在319份土样中，发现77份土样有微生物生长，并从中分离到70株酵母和10株细菌。通过内醚糖同化比较测试，发现Y215号菌的同化能力最强，在培养3天后，2%内醚糖的利用率达到了64.12%，经鉴定为斯达油脂酵母（Lipomyces starkeyi）。然而，在所有测试和分离的微生物中，没有发现既能同化又能发酵内醚糖为乙醇的菌株。本研究为通过遗传工程方法构建发酵内醚糖为乙醇的工程菌株提供了较好的菌种资源。

关键词: 中药, 中药, 结核分枝杆菌, 抗分支杆菌活性, 药物敏感性

Abstract:

The levoglucosan-assimilating and ethanol-producing microorganisms were screened using 1,6-anhydro-β-D-glucopyranose (levoglucosan) of cellulosic pyrolysis products as a sole carbon source from 89 strains of microorganisms (mostly producing ethanol) and 319 soil samples, respectively. 2 strains of yeasts (Sporobolomyces 1 and Rhodotorula 1) and 6 strains of moulds (Rhizopus 4 and Monascus 2) had weak levoglucosan-assimilating ability in 89 strains of microorganisms. 70 strains of yeasts and 10 strains of bacteria were isolated from the 319 soil samples. In all isolated microorganisms, the strain of No. Y215 showed the strongest levoglucosan-assimilating ability. After it was cultured for three days, the utilization rate of 2% levoglucosan was up to 64.12%. This strain was classified as Lipomyces starkeyi according to a taxonomic identification. Among all tested microorganisms, no microorganisms could ferment levoglucosan to ethanol. The results gave microbial strains for constructing recombinant strains to ferment levoglucosan to ethanol.