Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress

doi:10.11924/j.issn.1000-6850.casb17030159

Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (17): 19-23.doi: 10.11924/j.issn.1000-6850.casb17030159

Special Issue: 生物技术；烟草种植与生产

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Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress

Deng Jun^1,2, Zhou Tiean^1,2, Hu Jiajin^1,2, Chen Zongxing^1,2, Xie Jie^1,2

(¹College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128; ²Cell Mechanic and Biosensing Institute, Hunan Agricultural University, Changsha 410128)

Received:2017-03-24 Revised:2017-04-20 Accepted:2017-04-21 Online:2017-06-16 Published:2017-06-16

Abstract

Abstract: Positively charged poly(diallyldimethylammonium chloride) (PDADMAC)modified quartz crystal microbalance (QCM) was used for the real-time monitoring of the viscoelastic changes of Arabidopsis suspension cells and BY-2 cells when they were subjected to low temperature stress to compare cold tolerance of these two types of plant cells. The results showed that as the decrease of the temperature, the cell viscoelastic index (CVI) of both types of the cells decreased and the cells became harder, the lower the temperature, the larger the drop in CVI. Over the temperature drop range (25°C-5°C), the change in CVI for Arabidopsis cells is smaller than that of BY-2 cells, which is consistent with that Arabidopsis has better cold tolerance than that of Nicotiana tabacumL. Therefore, we propose to use CVI as a new parameter to indicate the cold tolerance of plant cells.

Deng Jun,Zhou Tiean,Hu Jiajin,Chen Zongxing and Xie Jie. Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress[J]. Chinese Agricultural Science Bulletin, 2017, 33(17): 19-23.

References

[1] 胡日生,曾中,史瑞甫,等.烟草耐冷性的苗期鉴定新方法[J].中国农学通报,2014(1):124-127.
[2] 郑帮,陈燕,褚艳霞,等.拟南芥HSP70基因启动子表达载体的构建及在烟草BY2细胞中的应用[J].上海师范大学学报:自然科学版,2013,42(5):492-498.
[3] Smallwood M, Bowles D J. Plants in a cold climate[J]. Philosophical Transactions of the Royal Society B Biological Sciences, 2002,357:831-847.
[4] ülo Niinemets. Does the touch of cold make evergreen leaves tougher?[J]. Tree Physiology, 2016,36(3):267-272.
[5] 裴惠娟,张满效,安黎哲.非生物胁迫下植物细胞壁组分变化[J].生态学杂志,2011,30(6):1279-1286.
[6] 简令成,王红.逆境植物细胞生物学[M].科学出版社, 2009:25-31,154-163.
[7] Xi J, Chen J, Garcia M, et al. Quartz crystal microbalance in cell biology studies[J]. J Biochip Tissue Chip,2013,S5:1-9.
[8] Thomas G, Burnham N A, Camesano T A, et al. Measuring the mechanical properties of living cells using atomic force microscopy[J]. JoVE (Journal of Visualized Experiments),2013(76):e50497,doi:10.3791/50497.
[9] Radoti?, Ksenija, Roduit, et al. Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth[J]. Biophysical Journal,2012,103(3):386-394.
[10] Zhou T, Zhou Z, Zhou S, et al. Real-time monitoring of contractile properties of H9C2 cardiomyoblasts by quartz crystal microbalance[J]. Analytical Methods,2015,8(3):488-495.
[11] 周铁安, Kenneth A.Marx, Susan J Braunhut. 石英微天平技术实时跟踪细胞-基质、细胞-细胞之间交互作用的动态变化[C].中国化学会学术年会第9分会场,2012.
[12] 周铁安,陈宗星,段海风,等.石英晶体微天平技术用于模式植物细胞力学性能的初步研究[C].全国生物流变学学术会议,2015.
[13] Geitmann A. Experimental approaches used to quantify physical parameters at cellular and subcellular levels.[J]. American Journal of Botany,2006,93(10):1380-90.
[14] Buer C S, Weathers P J, Swartzlander G A. Changes in Hechtian strands in cold-hardened cells measured by optical microsurgery[J]. Plant physiology,2000,122(4):1365-1378.
[15] Miura K, Furumoto T. Cold signaling and cold response in plants[J]. International journal of molecular sciences,2013,14(3):5312-5337.
[16] Rihan H Z, Al-Issawi M, Fuller M P. Advances in physiological and molecular aspects of plant cold tolerance[J]. Journal of Plant Interactions,2017,12(1):143-157.
[17] Houston K, Tucker M R, Chowdhury J, et al. The Plant Cell Wall: A Complex and Dynamic Structure As Revealed by the Responses of Genes under Stress Conditions[J]. Frontiers in Plant Science,2016(984):1-18.
[18] Sangwan V, Omann F, Dhindsa R S. Early steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity[J]. The Plant Journal,2000,23(6):785-794.
[19] Scholz F G, Bucci S J, Arias N, et al. Osmotic and elastic adjustments in cold desert shrubs differing in rooting depth: coping with drought and subzero temperatures[J].Oecologia,2012,170:885-897.
[20] Tenhaken R. Cell wall remodeling under abiotic stress[J]. Frontiers in plant science,2015,5:771.
[21] 孔琼,王云月,杨红玉,等.拟南芥悬浮细胞系培养条件优化[J].经济林研究,2013,31(1):115-118.
[22] 丁锐,朱延姝,崔震海,等.高等植物不同类型细胞的分离技术[J].科技通报,2012,28(1):80-85.
[23] Canale C, Petrelli A, Salerno M, et al. A new quantitative experimental approach to investigate single cell adhesion on multifunctional substrates[J]. Biosensors and Bioelectronics,2013,48:172-179.
[24] Zhou T, Marx K A, Dewilde A H, et al. Dynamic cell adhesion and viscoelastic signatures distinguish normal from malignant human mammary cells using quartz crystal microbalance[J]. Analytical Biochemistry,2011,421(1):164-171.
[25] 段海风.拟南芥粘弹性测定方法的建立[D].长沙:湖南农业大学,2015:24-25.
[26] 王柏,李志坚.植物脂肪酸与抗寒性关系研究进展[J].甘肃农业科技,2014(12):54-57.
[27] 吴广霞,唐献龙,杨德光,等.植物低温胁迫生理研究进展[J].作物杂志,2008(3):17-19.
[28] 杨毅,赵杨,朱果利.气孔运动中pH对保卫细胞壁弹性模量的影响[J].科学通报,2011,56(30):2523-2526.
[29] Peaucelle A, Braybrook S A, Le Guillou L. Pectin-induced changes in cell wall mechanics underlie organ initiation in Arabidopsis[J]. Current Biology,2011,21(20):1720-1726.

Viscoelastic Changes and Cold Tolerance Comparison of Arabidopsis and Tobacco Cells Under Low Temperature Stress

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