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    Apricot Initial Flowering Period and Meteorological Factors of Kashi, Xinjiang: Correlation and Predication
    Kerimu Abasi, Nu`erpatiman Maimaitireyimu, Meng Fanxue, Patiman Abuduaini, Zhang Qin
    Chinese Agricultural Science Bulletin    2021, 37 (1): 121-131.   DOI: 10.11924/j.issn.1000-6850.casb20200200099
    Abstract + (18553)    HTML (4686)    PDF (1314KB) (25273)      

    Based on the observation of the apricot tree phenology and the daily average temperature, minimum temperature, precipitation, and sunshine hours of the corresponding period in Kashi City, and Yecheng, Shache, Bachu and Maigaiti County of Kashi Prefecture of Xinjiang, the relationship between the beginning of apricot flowering and meteorological factors was analyzed using correlation analysis and regression analysis. The results show that among the various meteorological factors, the lowest temperature has the greatest impact on flowering, followed by the average temperature and sunlight, and the precipitation and other meteorological factors have less influence. The temperature in the study area is positively correlated to the flowering period of the apricot tree. From 1984 to 2019, the minimum temperature and average temperature increase in Kashi from late February to late March was 0.2-1.7℃/10 years. The apricot flower bud expansion period, flower bud opening period, the beginning of flowering was ahead of time by 5.3 to 8.7, 3.1 to 5.6, and 2.2 to 3.6 d/10 a, respectively. In apricot flowering period forecasting service, the daily average temperature of north, south and east of Kashi passed through ≥0℃ from day 1 to 34, day 39 to 41, day 37 to 39, or the accumulated temperature passed ≥0℃ before flowering reaching 250, 270-280, and 250-270℃, respectively, or the sliding average temperature of 5 days before the beginning of flowering passed≥12℃ could be used as the basis for predicting the beginning of apricot tree flowering.

    Variation Characteristics and Impact of Climate Factors During Winter Wheat Growth in Kashgar, Xinjiang
    Chinese Agricultural Science Bulletin    2016, 32 (27): 34-41.   DOI: 10.11924/j.issn.1000-6850.casb16010061
    Abstract + (17870)       PDF (1022KB) (24075)      
    The study aims to provide references for crop production in winter wheat area of Kashgar. The data of temperature, precipitation, sunshine duration from 1981 to 2014 during the growth period of winter wheat in Kashgar was used to study the variation characteristics of climate factors and their effects on winter wheat growth and development by the climate statistical method. The results indicated that the annual average temperature change during winter wheat growth in Kashgar showed an increasing trend. The precipitation showed a decreasing trend in the first two months after winter (March and April), and an obviously increasing trend in the rest time. Meanwhile, the effective accumulative temperature and the sunshine duration showed an increasing trend. Each development stage of winter wheat was ahead of schedule in different degrees during recent 34 years, especially the early stage and the jointing stage, but the overwintering stage showed a trend of delay. The temperature, effective accumulative temperature and the sunshine duration were negatively correlated with each development stage of the winter wheat. In short, the main reason for the advance of winter wheat growth period was the increase of temperature, effective accumulative temperature and sunshine duration. The impact of climate warming on winter wheat growth was significant in Kashgar.
    Jujube in Kashgar: Planting Meteorological Condition Analysis and Climate Quality Certification
    Chinese Agricultural Science Bulletin    2018, 34 (31): 119-124.   DOI: 10.11924/j.issn.1000-6850.casb17070145
    Abstract + (16970)            
    Based on sunshine duration, relative humidity, wind speed data from the national reference stations of Kashgar and the air temperature, precipitation from automatic meteorological station in Baishikeremu, we analyzed the effects of meteorological conditions and meteorological factors in Kashgar in 2015 on jujube planting and discussed the corresponding measures, studied the climatic suitability zoning indices and climatic conditions for jujube planting in Kashgar, in order to evaluate the climatic quality levels of jujube, determine the main climatic factors affecting jujube quality, and provide a basis for jujube planting techniques and production management. The results showed that: among the meteorological factors in Kashgar in 2015, sunshine was the most conducive to the growth and development of jujube and fruit coloring; there were influences of high temperature and precipitation on the growth and development period of jujube in 2015, and corresponding agricultural measures should be adopted to ensure jujube quality and yield. According to the grade of climatic suitability zoning index of jujube, the climatic conditions of jujube growth of the year and the production management of jujube enterprises, the climate quality grade of jujube in the certified region is excellent.
    The Stage Features and Policy Choices in China’s Agriculture Development A Comparative Analysis under the Perspective of“Four Stages Theory” in International Agricultural Development Process
    Chinese Agricultural Science Bulletin    2010, 26 (19): 439-444.   DOI: 10.11924/j.issn.1000-6850.2010-1489
    Abstract + (5177)       PDF (583730KB) (11576)      

    Accelerating agriculture’s modernization and internationalization process is an important strategy of Chinese agriculture’s development in the 21st century. The transition from traditional agriculture to modern agriculture worldwide is divided into four stages, namely, the period for investment in agriculture, the period for agricultural resources’outflow, the period for the integration of agriculture and macroeconomic, and the period for feeding agriculture. The four stages theory in lnternational agricultural development reflects the process of agricultural modernization, that is, in a transition process from the traditional business module to modern business module within a country. In the comparative analysis perspective, this paper attempts to use the four stages theory to understand the current stage and the main features of China's agricultural development of countries in recent years to understand a series of strategies and policies which have been implemented to support and benefit rural households, and to understand the long-term goal Agriculture for Development better.

    Studies on the Inversion of Soil Organic Matter Content Based on Hyper-spectrum
    Chinese Agricultural Science Bulletin    2013, 29 (23): 146-152.   DOI: 10.11924/j.issn.1000-6850.2012-3343
    Abstract + (5312)       PDF (921750KB) (10696)      
    The use of hyperspectral can conduct quantitative inversion on soil organic matter content, which is an important indicator of soil fertility, and then provide a reference for the rapid determination of surface soil organic matter content of accurate agricultural. The author conducted the spectral measurements on treated soil samples under laboratory conditions by using the spectroradiometer-U.S.ASD FieldSpec FR, established the multiple regression relationship model between the soil spectral variables and soil organic matter content through the correlation analysis between the different variations of the spectral reflectance of the soil samples and the organic matter content of the soil. The results showed that: the soil organic matter content inversed regression model, which was established by employing the first-order differential spectral reflectance at the wavelength of 492 nm, 663 nm, 1221 nm, 1317 nm, 1835 nm and 2130 nm, possessed the best prediction accuracy, the coefficient of determination R2 was 0.909. The established hyperspectral inversion model of the soil organic matter content could predict the soil organic matter content with the most accurate, and it also provide a new approach for the rapid determination of soil organic matter content of precision agriculture.
    Analysis of SUSIRI Gene from Rice by Bioinformatics and Subcellular Localization
    Lian Xiaohua and Chen Jian
    Chinese Agricultural Science Bulletin    2015, 31 (6): 128-135.   DOI: 10.11924/j.issn.1000-6850.casb14110097
    Abstract + (5963)       PDF (1114KB) (10673)      
    Transcription factors are a kind of proteins that play an important role in the regulation of plant growth and development, as well as in plant response to environmental variation. They usually contain a DNA-binding domain, a transcription regulation domain, an oligomerization site and a nuclear localization signal inside their DNA sequence. WRKY proteins had been identified as one of the important transcription factors in plants. There have been 103 WRKY genes which were predicted in rice genome by data mining and the annotation of the biological function of these genes had the important value for the functional genomics of rice. SUSIRI gene was one of the WRKY transcription factor genes cloned from the Oryza sativa sub. Japonica var. Nipponbare and its ORF was 1755 bp long, encoding a polypeptide of 584 amino acids with two typical WRKY domains. In this study, based on nucleotide sequence of the SUSIRI gene, its biological function in gene regulation in rice had been further elucidated. Firstly, the structure and functions of the predicted protein were analyzed by bioinformatics tools while the Motif sequence of SUSIRI gene was analyzed by using EBI interpro database, the conserved domains of the protein was analyzed by NCBI CDD database; the hydrophilicity (hydrophobicity) of amino acid residues and the transmembrane domains of SUSIRI protein were analyzed by DNAMAN and CBS TMHMM. By using CBS Protcomp version 9.0 and Protfun software, the subcellular localization and function of protein were predicted. Secondly, the fluorescence expression vector of pNSUGFP driven by actin promoter was constructed by fusing the SUSIRI gene and GFP gene and was used to transform into epidermis cell of onion by Gene gun for detecting gene subcellular location. The prediction from bioinformatics showed that the SUSIRI gene had the greatest probability in function of transcription, transcription regulation or signal transduction in the rice cell with the value in prediction of 0.973, 1.598 and 0.602. Similarly, it had the greatest probability in participation of translation, central intermediary metabolism, fatty acid metabolism with the predicted value of 4.800, 1.490 and 1.265. Because of high content of hydrophilic amino acid residues among peptide sequences, the SUSIRI protein had hardly inserted into the membrane in the cell when it was likely to be localized within the cell nucleus since the strong nucleus location signal was detected from the sequence of amino acids. By using the fluorescence microscopy to observe the onion epidermic cells, the GFP protein was shown to express along the cell wall in the control, but the fused protein of SUSIRI-GFP expressed strongly in the cell nucleus. The result indicated that the SUSIRI gene was likely to be the transcription factor regulating the intermediary metabolism in rice and playing the role during the gene transcription. SUSIRI protein could be localized in nucleolus which is an obvious feature of transcription factor of SUSIRI gene determined by the experiment.
    Olive in Wudu Mountain: Growth Meteorological Condition and Suitable Climate Division
    Chinese Agricultural Science Bulletin    2016, 32 (31): 161-166.   DOI: 10.11924/j.issn.1000-6850.casb16020080
    Abstract + (4766)       PDF (859KB) (10266)      
    The paper aims to illustrate the key growth stages of olive and the relationship between botanical characteristics and meteorological variables. In Wudu mountain, the botanical characteristics of olive and the meteorological observation data from 2012 to 2015 were collected. Based on the leading indicator of average temperature in January and several other factors such as annual average temperature, annual average relative humidity, and annual sunshine hours, an olive suitable climate division index system was built for Wudu mountain. The fine climate division map for olive cultivation suitability was made by applying GIS technology. The results indicated that: the most suitable olive planting areas were located in the valley plains, basins, and shallow mountainous areas below the elevation of 1300 m in the southern Wudu and Bailong river basin; suitable olive planting areas were located in the valley plains, basins, and shallow mountainous areas in the elevation of 1400- 1500 m; the other areas were unsuitable for olive growing which were mainly in the northwest Wudu and other places above the elevation of 1500 m. The results can guide scientific planning and rational arrangement for Wudu mountain and make the best of climate resources to develop olive industry, and also provide a scientific basis for prevention and mitigation of meteorological disasters.
    The screening and identification of antagonistic bacteria against Penicillium expansum and the inhibitive effects of fermentation broth
    Chinese Agricultural Science Bulletin    2010, 26 (5): 8-13.   DOI: 10.11924/j.issn.1000-6850.2009-2249
    Abstract + (8368)       PDF (4247140KB) (8643)      

    Dozens of strains of bacteria were isolated from the surface of fruits and the soil, they were selected through the plate confrontation method and six strains which have good antagonistic effect are obtained, among them two strains named T3 and T8 which the fermentation broth showed a significant antagonistic effect to Penicillium expansum are found. The antifungal activity of fermentation broth of T3 and T8 are studied. The stability experiment show that after treatment with 100 ℃ and two proteases, the fermentation broth of T3 performs good antagonistic effect, while antagonistic effect of the fermentation broth of T8 decreases a lot. The antifungal activity of T3 and T8 reach almost highest level after 2 days growth. It is also found that BPA was the optical medium to T3 and PDA was the optical medium to T8. The fermentation broth of two strains can cause hyphae deformity of Penicillium expansum. According to their morphological, physiological, biochemical characters, and the analysis of the sequence of 16SrDNA,T3 and T8 were preliminary identified as Bacillus subtilis, two strains of the similarity between the gene sequence is 93.45%.

    Progress of Study on Salicylic Acid’s Physiological Role in Plant
    Chinese Agricultural Science Bulletin    2010, 26 (15): 207-214.   DOI: 10.11924/j.issn.1000-6850.2010-0170
    Abstract + (5677)       PDF (634899KB) (6100)      

    Salicylic acid is one of the ubiquitously endogenous signal molecules in plant, which has been recognized as a kind of hormones, and has great physiological function such as disease resistance, low temperature resistance, drought resistance, salt resistance, ultraviolet radiation resistance, fruit maturity, gardening product preservation and so on. This article summarized the recent research progress of salicylic acid (SA)’s physiological effects on plants.

    Ecological Sensitivity Analysis on Wanquan River Watershed Based on GIS
    Chinese Agricultural Science Bulletin    2012, 28 (10): 69-73.   DOI: 10.11924/j.issn.1000-6850.2011-3252
    Abstract + (1839)       PDF (656986KB) (5537)      

    In order to understand the ecological problems in Wanquan River watershed, the author selected 5 ecological sensitive evaluation factors, including slope degree, elevation, soil type, water buffer and land use type to analyze ecological sensitivity comprehensively based on GIS, using the survey material and related research data. The results indicated: (1) low sensitive areas (38.69%)>non-sensitive areas (26.7%)>moderately sensitive areas (22.84%)>highly sensitive areas (8.38%)>extremely sensitive areas (3.39%); (2) The extremely sensitive areas were mainly distributed the upper reaches of catchment, the highly sensitive areas were scattered the all the watershed, moderately sensitive areas were mainly distributed the upper-middle reaches, the low sensitive and non-sensitive areas were concentrated in the lower-middle reaches of watershed. This demonstrated that the spatial distribution of ecological sensitivity of Wanquan River basin was significantly different, the ecological sensitivity was low, the ecological environment had good condition.

    Study the effects of Soil Improvement on Acid Soil in the South of China
    Chinese Agricultural Science Bulletin    2009, 25 (20): 160-165.   DOI: 10.11924/j.issn.1000-6850.2009-1086
    Abstract + (1659)       PDF (616615KB) (5476)      

    According to the problem of soil sourness in the South of China, in a pot experiment, 8 kinds of soil modifier were selected as soil improvement measures. The effects of different soil modifier on the characteristic of sorghum growth、Soil Physical Properties and Soil Chemical Properties was studied. On the basis of the interaction among sorghum, soil and modifier, discussed the mechanism of action of modifier, and revealed variation of the properties of acid soil under the action of modifier. The result indicated that the desulphurization ash modifier and the organic fertilizer had good function to the sorghum growth, Soil bulk density of acid soil which was applying humic acid, the humic acid + lime, XP2, the organic fertilizer and the desulphurization ash modifier, decreased in some degree compared with CK; But results of lime,XP1 and market modifier were opposite. After the Duncan multiple comparisons, there was significant difference between application of modifier ( except for lime treatment) and CK(P<0.05). The soil pH with XP1 or the lime increased remarkably by 2 units compared with CK, the difference assumes the remarkable level(P<0.05). The employment modifier might also alleviate the acid soil aluminum poisonous harm effectively, the laterite, the red laterite lime treatment reduces 18.0%, 18.7%, XP1 processing compared to the comparison to reduce 16.4% separately, 16.0%.

    Effects of Different Fruit-Setting Methods on the Endohormone Contents and fruit setting rate in the Florescence Period of Dongzao (Zizyphus jujuba Mill. cv. Dongzao)
    Ma Qinghua1,2, Xu Jiuru2, Wang Guixi1, Yao Lixin2
    Chinese Agricultural Science Bulletin    2010, 26 (9): 234-238.   DOI: 10.11924/j.issn.1000-6850.2009-2759
    Abstract + (3236)       PDF (662264KB) (5309)      

    In this study, different fruit-setting methods such as girdling, tying wire and spraying GA3 were used in the florescence period of Dongzao (Zizyphus jujuba Mill. cv. Dongzao). The effects of those methods on both the endohormone contents (IAA、ABA、GA、ZR) in leaves, blooming flowers, young fruits, flower-buds, shatters and the fruit-setting rate were analysised in order to discuss the function of different fruit-setting methods. The result showed that, girdling and spraying GA3 could effectively improve the balance of endohormone contents in Dongzao trees, integrating the two methods could improve the fruit-setting rate of Dongzao evidently. The effect of tying wire on improving the fruit-setting rate was not as good as girdling and the effect on the endohormone contents had not obvious difference with control. At the same time, there were some shortcomings in the method of tying wire, so that this method could not be used on Dongzao.

    Impacts of Climate Change on the Agricultural Planting Structure of Main Crops in Hunan
    Chinese Agricultural Science Bulletin    2010, 26 (24): 276-286.   DOI: 10.11924/j.issn.1000-6850.2010-1506
    Abstract + (5267)       PDF (11595038KB) (5251)      

    Based on daily meteorological records of mean, minimum and maximum air temperatures, sunshine hours as well as precipitation from more than 97stations from 1961 to 2008 across Hunan province, we employ the best small grid interpolating methods to develop the high-resolution grid series data with the grid distance of 500 m. Based on the main crops growth conditions and regionalization research results of double cropping rice, rape, cotton, satsuma mandarin, sweet orange, pummelo, camellia oleifera, tobacco, we establish the corresponding indexes of climatic regionalization for main crops suitability. Combined with the geographic information data and based on GIS, we carry out the work of dynamic climatic regionalization for main crops planting suitability. The results show that the climate change has some influences on the planting structure of main crops in Hunan province. The major impact on double cropping rice is the change of ripe cycle, onrape and camellia oleifera as well asorange is the increased area of optimum suitable planting, on cotton and tobacco is the decreased area of optimum suitable planting. According to the impacts of climate change on planting structure and the results of agricultural climatic regionalization, we propose as follows: make full use of climate resources of lakeshore plain and valley plain as well as basin to raise multiple crop index, and to increase the yield per unit area; make full use of mountain climate resources to make the camellia oleifera industry bigger and stronger; make full use of regional climate resources to create the orange brand; make full use of microclimatic resource to develop the special tobacco planting in western and southern Hunan province

    Determined Methods of Chlorophyll from Maize
    Li Dexiao, Guo Yuexia, Yun Haiyan, Zhang Min, Gong Xiaoyan, Mu Fang
    Chinese Agricultural Science Bulletin    2005, 21 (6): 153-153.   DOI: 10.11924/j.issn.1000-6850.0506153
    Abstract + (6147)       PDF (1197488KB) (5211)      
    The absorption spectrums and extraction efficiencies of chlorophyll from maize seedling after frozen treatment were compared on six determined methods using different organic solvents. The results showed that the absorption spectrums of five solutions were identical with that of acetone solution, so Arnon’s formula of chlorophyll was suitable for other methods. In house temperature, soaking methods were better than Arnon method. The extraction efficiencies of chlorophyll using mixture solution better than using acetone or ethanol alone, methods of acetone mixed ethanol were preferable for fast extraction and stability. Soaking methods in 50℃ after frozen treatment could accelerate extraction of chlorophyll, but also speed up degradation of chlorophyll, so light screening was necessary.
    Study on Identification and Fermentation Condition of the Strain ZY-19-2 which Inhibit to Tobacco Black Shank
    Chinese Agricultural Science Bulletin    2011, 27 (7): 257-261.   DOI: 10.11924/j.issn.1000-6850.2010-1874
    Abstract + (4926)       PDF (1161994KB) (5157)      

    Tobacco black shank is one of the most serious diseases of tobacco. People mainly adopt integrated management to the disease like, breeding for resistance varieties, agro-protection, chemical control, biological control and plant induced resistance in production of tobacco. Biological control is the best way against the disease for tobacco. The strain ZY-19-2 was isolated and screened from rhizosphere of tobacco, which demonstrated high inhibitory to tobacco black shank. In order to appraise its value, we identified the strain and studied its activity of Chitinase in different culture conditions. The results indicated that the stain ZY-19-2 was Paecilomyces lilacinus, which showed strongly inhibition action to parasitica Phytophthroa Dast var. nicotianae and the highest activity of Chitinase was 0.216 U/mL using 1.2% chitin as carbons source in the medium and the initial pH of fermentation fluid was 6.0, with 1% peptone as nitrogen, 0.1% Tween-80 as a surfactant, fermentation time being 60 h, rotation speed of 120 min. The strain ZY-19-2 was optimized in culture different conditions, which established foundation on producing highly effective Chitinase and chitooligosaccarides and controlling tobacco black shank.

    Studies on the Induction of Stenospermocarpy in Litchi of Different Embryonic Development Cultivars
    Chinese Agricultural Science Bulletin    2013, 29 (16): 115-118.   DOI: 10.11924/j.issn.1000-6850.2012-3435
    Abstract + (2011)       PDF (512005KB) (5139)      
    To develop a technique for improving the ratio of abortive seeds and quality, the normal seed cultivar ‘Heiye’ and partial abortive seed cultivar ‘Lingshan Xiangli’ were treated with different concentration of MH (maleic hydrazide) and some other plant growth regulators such as 2,4-D, BONA or CPPU to study the effect to the ratio of abortive seeds and yield. The results showed that the ratio of abortive seeds was increased with the increasing of MH concentration, but the yield was decreased simultaneously. The suitable concentration of MH was 600-800 mg/L in the induction of stenospermocarpy. 2,4-D and MH was the best treatment in inducing stenospermocarpy in this study. The reaction to the plant growth regulators was different between ‘Heiye’ and ‘Lingshan Xiangli’. The ratio of induced abortive seed in ‘Lingshan Xiangli’ was 89.77%, only was 8.79% in ‘Heiye’.
    Principle and Methodology of QTL Analysis in Crop
    Xi Zhangying
    Chinese Agricultural Science Bulletin    2005, 21 (1): 88-88.   DOI: 10.11924/j.issn.1000-6850.050188
    Abstract + (5488)       PDF (1242830KB) (4381)      
    Most agronomic traits are quantitative traits. Mapping of quantitative trait loci (QTL) is based on Morgan linked inheritance principle. QTL mapping populations include the primary mapping population, the secondary mapping population and the advanced mapping population. Methods of QTL analysis include the single marker mapping, the interval mapping, the composite interval mapping and the mixed linear model. The precise of QTL mapping are affected by the population size, analysis method, QTL distribution and so on. Some disadvantages are found in traditional mapping populations and analysis methods, so new mapping populations and new methods are need in future QTL analysis.
    Effects of Biochar on Soil and Fertilizer and Future Research
    Chinese Agricultural Science Bulletin    2011, 27 (15): 16-25.   DOI: 10.11924/j.issn.1000-6850.2010-3470
    Abstract + (3904)       PDF (676394KB) (4115)      

    In order to boost the study and agricultural application of biochar in China, in the article, the research literatures and findings about biochar were retrospected and reviewed, and future research issues were suggested, the summaries were as follows: biochar was a solid byproduct derived from biomass pyrolysis in low or zero oxygen environment, highly organic carbon, porous, alkaline, highly capability of adsorption, multi-propose materials. Biochar was an excellent soil amendment which could increase soil organic carbon content, improve soil water-holding and nutrient-retention capacity, reduce nutrient losses from soils, and was beneficial to inhabit and activity of microbes in soils, especially for mycorrhizal fungi (MF). Biochar alone increased crop growth and yield in specific circumstances, but the effects of the co-application or combination of biochar with fertilizers on crop growth and yield showed positive in nearly all soils, which was attributed to reciprocal or synergistic effects of both fertilizer and biohcar because of fertilizer alleviating nutrient deficiency of biochar itself which endowed fertilizer with nutrient slow release characteristics. Biochar delayed nutrient release of fertilizer to soil, reduced nutrient losses from fertilizer and soil, increased nutrient utilizing efficiency, and was efficient-promoting carrier to fertilizer. Biochar was a carbon-sequestrating carrier which was extremely stable in soil, and could sequestrate carbon in soils for hundreds to thousand years. The multi-win technology was the thermal conversion of biomass wastes into biochar which was used to agriculture. However widely and deeply studies should be given to many issues about biochar. Thus, strong emphasis will given to basic and applied research on biochar in order to boost biochar production and agricultural use, finally agricultural ‘black revolution’ in 21st century.

    Analysis and Study of Variety Quality Appraisement in Waxy Maize Regional Trials in Huanghuaihai Region
    Chinese Agricultural Science Bulletin    2009, 25 (8): 127-131.   DOI: 10.11924/j.issn.1000-6850.2008-1322
    Abstract + (2666)       PDF (482798KB) (4114)      
    According to analysis of basal instance of 31 varieties quality appraisement in waxy maize regional trials in huanghuaihai region in 2006-2007,it was summarized that waxy trait and depth of pericarp was main attack quality aims that remarkably better or better waxy maize would be bred than the contrast. According to analysis of main method and procedure and technology of quality appraisement, the experience was summarized that waxy maize quality was scientifically and impersonally and justly and well and truly appraised .At the same time, the proposal of quality appraisement improved was tabled.
    Comparing Study on several Methods for DNA Extraction from endophytic fungi
    Wu Fahong, Huang Dongyi, Huang Xiaolong, Zhou Xin, Cheng Wenjie
    Chinese Agricultural Science Bulletin    2009, 25 (8): 62-64.   DOI: 10.11924/j.issn.1000-6850.2009-0284
    Abstract + (3595)       PDF (721300KB) (4095)      
    In this study, three different methods were used to extract DNA from endophytic fungi: CTAB,SDS and the improved CTAB. Quality and quantity of the extracted DNA were compared for the there methods. The results showed that all of the there methods could obtain the DNA, but the improved CTAB could obtain the highest quantity of the purest fungal DNA. The purest DNA were amplified by PCR .The clear and singal gel fragment showed that the extracted DNA could usde for the other biological experiments.