Abstract: [Objective] The study in order to provide a theoretical basis for scientific fertilization which by means of analyzes effects of nutrient elements on drought resistance of Pinus tabulaeformis. [Methods] the test set 5 treatment as S1 (routine control), S2 (clean water control), S3 (N), S4 (NK), S5 (NPK) and repeat 3 times. [results] The S5 treatment SOD activity that was higher than S1 treatment 68.66%,64.54%,36.02%,30.03% when in May, June, July and August and then had significant difference; POD activity that S5 was higher than S1 treatment as 79.96%,117.82%,51.65%,41.28% and then had significant difference; CAT activity S5 was higher than S1 treatment as 35.86%,33.51% when in June and August and then had significant difference; PPO activity S5 was higher than S1 treatment as 92.18%,125.15%,74.95%,57.77% and then had significant difference; The conductivity of S5 was always at lowest level, at the same times, it was lower than control(S1)treatment 40.46%,24.73%,25.99%,55.55% and then had significant difference; [Conclusion] S5 was the best treatment as to enhance the activity of protective enzymes and decrease the conductivity of Pinus tabulaeformis seedlings under water stress.