Abstract: In order to identify the potato varieties, we used polyacrylamide gel electrophoresis to separate the peroxidase isozyme of the potato, and identify the potato varieties with the enzyme band map. With 14 different varieties of potato buds, stems and leaves as experimental material. Respectively to extract peroxidase isozyme of the different parts of the experimental materials for polyacrylamide gel electrophoresis. Analysis of isoenzyme zymogram differences, to determine the differences between the varieties and genetic relationship. The results showed that the peroxidase isozyme of buds could be used as a criterion to distinguish the potato varieties. In 14 different varieties, No.4 and No.7 enzymatic bands were similar, they were Jinshu 14 and Jinshu 11; No.6 and No.9 enzymatic bands were similar, they were Jinshu 21 and Jinshu 18; No.5 and No.12 enzymatic bands were similar, they were Tongshu 22 and Tongshu 20; No.13 and No.14 enzymatic bands were similar, they were 05-32-7 and Tongshu 23. It indicated that the genetic relationships between these cultivars were relatively close.