The Effect of Matricaria chamomilla Essential Oil on Skin Wound Healing

doi:10.11924/j.issn.1000-6850.casb18110096

Abstract

Abstract:

This study aims to investigate the effects of Matricaria chamomilla essential oil on the proliferation and migration of fibroblasts and the wound healing in mice. The effects of different concentrations of M. chamomilla essential oil on the proliferation of fibroblasts were detected by CCK8 method, while the effects on the migration of fibroblasts were detected by scratch method. Excision wound model was established to evaluate the effects of M. chamomilla essential oil on the wound healing in mice. The results showed that: M. chamomilla essential oil significantly promoted the proliferation and migration of fibroblasts in a dose-dependent manner. Meanwhile, it significantly improved the wound healing rate of mice skin. The number of fibroblasts and the thickness of dermis of the wound skin in essential oil treatment group increased significantly compared with that in the control group. These results indicated that M. chamomilla essential oil could promote skin wound healing, and its mechanism might be related to promoting fibroblast proliferation and migration.

Key words: Matricaria chamomilla essential oil, wound healing, fibroblasts, excision wound model

CLC Number:

R285.5

Shen Junlian, Zhang Nan, Li Yuhong, Yao Lei, Li Shuangyue. The Effect of Matricaria chamomilla Essential Oil on Skin Wound Healing[J]. Chinese Agricultural Science Bulletin, 2020, 36(4): 67-75.

Figures/Tables 8

References 27

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编号	化学成分		相对百分含量/%
编号	中文名	英文名	相对百分含量/%
1	反式β-罗勒烯	trans-β-Ocimene	0.5807
2	β-罗勒烯	β-Ocimene	4.1890
3	蒿酮	ArteMisia ketone	0.2388
4	榄香烯异构体	Elemene isomer	0.8598
5	α环异戊二烯	α-Isocomene	0.3354
6	β-榄香烯	β-Elemene	0.3064
7	β-石竹烯	β-Caryophyllene	0.5165
8	α-律草烯	α-Humulene	0.1634
9	(E)-β-金合欢烯	(E)-β-Farnesene	19.1319
10	脱氢倍半桉叶素	Dehydrosequicineole	0.2322
11	右旋大根香叶烯	Germacrene D	9.8802
12	(Z,E)-α-金合欢烯	(Z,E)-α-farnesene	1.3652
13	双环大根香叶烯	Bicyclogermacrene	7.9543
14	α-金合欢烯	α-Farnesene	13.4396
15	(+)-δ-荜澄茄烯	(+)-δ-Cadinene	0.3832
16	橙花叔醇	Nerolidol	0.7125
17	蓝桉醇	Globulol	0.5952
18	斯巴醇	Spathulenol	1.0679
19	绿花白千层醇	Viridiflorol	0.3488
20	β-桉叶油醇	β-Eudesmol	0.1845
21	澳白檀醇	Lanceol	0.2245
22	τ-荜澄茄醇	τ-Cadinol	0.2903
23	α-氧化红没药醇 B	α-Bisabolol oxide B	4.3743
24	香橙烯氧化物	Aromadendrene oxide	0.304
25	氧化红没药酮 A	Bisabolone oxide A	7.3854
26	母菊薁	Chamazulene	7.9475
27	α-氧化红没药醇 A	α-Bisabolol oxide A	4.4495
28	2-(己-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hexa-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	9.9377
29	2-(庚-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hepta-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	0.5699
30	二十五烷烃	Pentacosane	0.2634

编号	化学成分		相对百分含量/%
编号	中文名	英文名	相对百分含量/%
1	反式β-罗勒烯	trans-β-Ocimene	0.5807
2	β-罗勒烯	β-Ocimene	4.1890
3	蒿酮	ArteMisia ketone	0.2388
4	榄香烯异构体	Elemene isomer	0.8598
5	α环异戊二烯	α-Isocomene	0.3354
6	β-榄香烯	β-Elemene	0.3064
7	β-石竹烯	β-Caryophyllene	0.5165
8	α-律草烯	α-Humulene	0.1634
9	(E)-β-金合欢烯	(E)-β-Farnesene	19.1319
10	脱氢倍半桉叶素	Dehydrosequicineole	0.2322
11	右旋大根香叶烯	Germacrene D	9.8802
12	(Z,E)-α-金合欢烯	(Z,E)-α-farnesene	1.3652
13	双环大根香叶烯	Bicyclogermacrene	7.9543
14	α-金合欢烯	α-Farnesene	13.4396
15	(+)-δ-荜澄茄烯	(+)-δ-Cadinene	0.3832
16	橙花叔醇	Nerolidol	0.7125
17	蓝桉醇	Globulol	0.5952
18	斯巴醇	Spathulenol	1.0679
19	绿花白千层醇	Viridiflorol	0.3488
20	β-桉叶油醇	β-Eudesmol	0.1845
21	澳白檀醇	Lanceol	0.2245
22	τ-荜澄茄醇	τ-Cadinol	0.2903
23	α-氧化红没药醇 B	α-Bisabolol oxide B	4.3743
24	香橙烯氧化物	Aromadendrene oxide	0.304
25	氧化红没药酮 A	Bisabolone oxide A	7.3854
26	母菊薁	Chamazulene	7.9475
27	α-氧化红没药醇 A	α-Bisabolol oxide A	4.4495
28	2-(己-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hexa-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	9.9377
29	2-(庚-2, 4-二炔-1-叉)-1, 6-二氧螺[4, 4]-3-烯	2-(Hepta-2,4-diyn-1-ylidene)-1,6-dioxaspiro[4.4]non-3-ene	0.5699
30	二十五烷烃	Pentacosane	0.2634

天数/d	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
1	4.14±2.48	5.84±3.84	8.51±3.00^*	7.00±2.60^*	7.58±2.97^*	5.83±3.79
4	26.71±3.66	27.57±4.65	37.39±4.91^*	35.82±5.03^*	39.40±4.16^*	28.90±4.32
7	37.99±2.80	38.92±3.34	51.57±3.07^*	49.33±2.17^*	50.64±4.16^*	45.77±2.54^*
10	86.48±5.84	86.54±6.49	93.99±2.80^*	92.52±3.18^*	95.11±2.79^*	92.79±3.29^*
14	93.31±3.03	93.69±4.78	97.59±0.95^*	96.29±1.67^*	97.04±0.77^*	97.69±0.91^*

天数/d	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
1	4.14±2.48	5.84±3.84	8.51±3.00^*	7.00±2.60^*	7.58±2.97^*	5.83±3.79
4	26.71±3.66	27.57±4.65	37.39±4.91^*	35.82±5.03^*	39.40±4.16^*	28.90±4.32
7	37.99±2.80	38.92±3.34	51.57±3.07^*	49.33±2.17^*	50.64±4.16^*	45.77±2.54^*
10	86.48±5.84	86.54±6.49	93.99±2.80^*	92.52±3.18^*	95.11±2.79^*	92.79±3.29^*
14	93.31±3.03	93.69±4.78	97.59±0.95^*	96.29±1.67^*	97.04±0.77^*	97.69±0.91^*

项目	空白组	对照组	rhEGF组	低浓度德国洋甘菊精油组	中浓度德国洋甘菊精油组	高浓度德国洋甘菊精油组
成纤维细胞数目	61.42±4.94	67.93±5.67	94.63±9.33^*	80.37±5.51^*	88.45±6.25^*	83.26±6.73^*
炎性细胞数目	45.43±5.78	42.59±3.86	35.39±6.02^*	38.67±4.38	34.42±5.21^*	31.53±3.44^*