The rice varieties Xian you 63, Fu liang you 2186, Dong nan 201, Min zi xiang were acted as experiment material, which were spraied with different concentrations of Se and Zn fertilizer during first heading date and full heading date. The results indicated that: (1)there were no significant difference in the plant height, panicle length, panicle numbers, grains per panicle and 1000-grain weight among different treatments, but there was significant difference in the fertility. (2)The yield per plant which were spraied by various concentrations of Se and Zn fertilizer were weightier than CK. Thereinto, the yield per plant of Fu liang you 2186 and Min zi xiang were respectively 14.03% and 15.65% weightier than CK, when they were spraied by the treatment of(Na2SeO3)22.2mg/kg+(ZnSo4)50mg/kg; The yield per plant of Xian you 63, when it was treated by Na2SeO3 22.2mg/kg, was 18.42% weightier than CK; The yield per plant of Dong nan 201 was 17.65% weightier than CK, when it was spraied with Na2SeO3 44.4mg/kg. (3) The concentration of Se fertilizer that ranged from 0 to 44.4mg/kg, the content of Se in brown rice was significantly increased, with increasing of the concentration of Se fertilizer. The content of Se in brown rice under the six treatments were respectively 3.43、8.29、16.86、4.14、7.71 and 17.36 times same as CK, but there were no significant difference in the content of Zn、Fe、Ca in brown rice among all treatments.