This paper studied the large quantity to rearing in artificial glasshouse and release in the tobacco field, The results showed that the inoculate parasite number and the aphid number when inoculating were important factors during the rearing of Aphidius gifuensis; The effect that creature prevented and cured amount to 89.3% in the tobacco field, the prevent and cure is obvious effect; It is mid-May aphid few that puts Aphidius gifuensis in the field, the same time prevent and cure result is as increase soon as times that put Aphidius gifuensis and number of put Aphidius gifuensis, the effect that prevent and cure is more and more good.