ISSR assessment of genetic variations in 30 varieties of banana(Musa spp.)was carried out .Eight primers were screened from 60 arbitrary primers, and a total of 54 DNA bands were amplified,among which 44(81.5%)were polymorphic. The average number of DNA bands amplified by each primer was 6.75,and the average genetic distance among 30 varieties was 0.313. Based on UPGMA cluster analysis of 54 DNA bands amplified by 8 primers a DNA molecular dendrogram was established,which divided the 30 varieties into four groups: group A, B, C and D.