Seedling stems with lateral buds were used as explants to study the shoot induction and propagation in Japanese W. foribumda under different tissue culture condition. An efficient plant regeneration system from explants of Japanese wisteria has been established in this study. The optimal medium for the induction of multiple shoots at the first stage of culture is MS medium supplemented with BA2mg/·L-1 BA, NAA0.1mg/·L-1 NAA, and 3.5% sucrose. High frequency of further multiplication were obtained in the medium of MS containing BA 2mg/·L-1 BA, IBA 0.5mg/·L-1 IBA, and 3% sucrose (pH 5.8~6.0), under cool white fluorescent light of 1500~2000Lx at (25±1)℃. The proliferation ratio was 2.8~3.6 at intervals of 25~30 days. The rooting rate in the medium of 1/2 MS supplemented with 0 ABT0.5mg/·L-1 ABT, IBA 0.5mg/·L-1 IBA, and 2.5% sucrose could reach over 95%. The regenerated plantlets were transplanted successfully in mixture soil with sand: peat: husk ashes (2:1:1) with survival rate of 86.5%.
