The main factors which influence the callus induction，differentiation, rooting and transplant of Chinese cephalotaxus sinensis from stem apex and spire studied. The plant regeneration system has been established． The results showed that the culture medium MS + 6–BA + NAA +2,4-D, temperature 28℃, the relative humidity 70%, and the illumination time 8h/d are better. Callus appears after three weeks. Sprouts per callus could be obtained in SH medium containing 0.1mg/L 2,4-D. The optimum condition for rooting was 1/2MS+1.0mg/L NAA.
