Three pretreatment methods for the determination of Tautine in marine products were compared ,which were the extraction with 6% sulfosalicyclic acid(SSA) in hot water bath, the extraction with 0.02mol/L hydrochloric acid immerse(HAI) and the extraction with 70% ethyl alcohol circumfluence(EAC)(hot-water bath). The results showed that SSA gave the most accurate determined values, the highest repeatability, recovery rate up to 98.17% and CV 1.86%. The SSA was low in reagent cost and easy to operate. Eighteen kinds of free amino acid could be determined at the same time with SSA, Which is the suitable way for the determination of Taurine analysis of marine products.