Research Progress on Microbial-Mediated Methane Oxidation and Its Prospects in Aquaculture

doi:10.11924/j.issn.1000-6850.casb2025-0756

Abstract

Abstract:

As an important greenhouse gas, methane (CH₄) emission contributes significantly to global warming, and methane emission reduction is important for mitigating the greenhouse effect. The aquaculture pond, as a typical shallow water ecosystem, is an easily overlooked source of methane emission. Pond sediments are rich in exogenous organic matter, which is the key area for carbon and nitrogen biogeochemical cycling through microbially mediated carbon and nitrogen cycle coupling. However, there is still a lack of systematic knowledge about microbially driven methane oxidation processes and their regulatory mechanisms in aquaculture ponds. This paper systematically reviewed the research progress of aerobic and anaerobic methane oxidation, focused on the community composition, metabolic mechanism, ecological distribution characteristics of methane-oxidizing microorganisms and their environmental influences, and summarized the methods for determining the potential rate of methane oxidation. On this basis, the key directions for future research in this field are proposed in view of the special characteristics of aquaculture pond ecosystems, aiming to provide scientific basis for methane emission reduction and green sustainable development in aquaculture.

Key words: aerobic methane oxidation, anaerobic methane oxidation, indoor incubation, aquaculture ponds

CLC Number:

S917.1水产微生物学

ZHANG Hongfei, XU Huimin, LI Dandan, LU Shangming, FANG Longxiang, LIU Zhuping, YANG Zhen, GAO Yuhang, ZHANG Li, SONG Chao, MENG Shunlong. Research Progress on Microbial-Mediated Methane Oxidation and Its Prospects in Aquaculture[J]. Chinese Agricultural Science Bulletin, 2026, 42(11): 202-210.

Figures/Tables 4

References 61

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类型	门（纲）	属（种）	特征
Ⅰ型（Ⅹ型）	γ-变形菌纲	甲基单胞菌属(Methylomona)、甲基球菌属(Methylococcus)、甲基杆菌属(Methylobacter)、甲基微菌属(Methylomicrobium)、Methylosphaera、甲基热菌属(Methylothermus)、Methylohalobius、甲基暖菌属(Methylocaldum)、甲基八叠球菌属(Methylosarcina)、Methylosoma	通过颗粒型甲烷单加氧酶氧化底物，作用底物多为低碳烃，在低甲烷浓度中适应性强
Ⅱ型	α-变形菌纲	甲基弯曲菌属(Methylosinus)、甲基胞囊菌属(Methylocystis)、甲基帽菌属(Methylocapsa)、Methylocella、Methyloferula	通过可溶性甲烷单加氧酶氧化底物，底物范围广，对于环境变化抵抗力更强
Ⅲ型	疣微菌门	Methylokorus infernorum、Acidimethylosilex fumarolicum、 Methyloacida kamchatkensis	分布于极端环境中

类型	门（纲）	属（种）	特征
Ⅰ型（Ⅹ型）	γ-变形菌纲	甲基单胞菌属(Methylomona)、甲基球菌属(Methylococcus)、甲基杆菌属(Methylobacter)、甲基微菌属(Methylomicrobium)、Methylosphaera、甲基热菌属(Methylothermus)、Methylohalobius、甲基暖菌属(Methylocaldum)、甲基八叠球菌属(Methylosarcina)、Methylosoma	通过颗粒型甲烷单加氧酶氧化底物，作用底物多为低碳烃，在低甲烷浓度中适应性强
Ⅱ型	α-变形菌纲	甲基弯曲菌属(Methylosinus)、甲基胞囊菌属(Methylocystis)、甲基帽菌属(Methylocapsa)、Methylocella、Methyloferula	通过可溶性甲烷单加氧酶氧化底物，底物范围广，对于环境变化抵抗力更强
Ⅲ型	疣微菌门	Methylokorus infernorum、Acidimethylosilex fumarolicum、 Methyloacida kamchatkensis	分布于极端环境中

类型	电子受体	反应式	吉布斯自由能/(kJ/mol)
好氧型	氧气(O₂)	CH₄+2O₂→CO₂+2H₂O	-858.7
硫酸盐还原型	硫酸盐(SO₄²)	CH₄+SO₄^2-→HCO₃^-+HS^-+H₂O	-16.6
反硝化型	硝酸盐(NO₃^-)	CH₄+4NO₃^-→CO₂+4NO₂^-+2H₂O	-483.4
反硝化型	亚硝酸盐(NO₂^-)	3CH₄+8NO₂^-+8H⁺→3CO₂+4N₂+10H₂O	-928.0
金属还原型	铁离子(Fe³⁺)	CH₄+8Fe³⁺+3H₂O→HCO₃^-+8Fe²⁺+9H⁺	-418.3
	锰离子(Mn⁴⁺)	CH₄+4MnO₂+7H⁺→HCO₃^-+4Mn²⁺+5H₂O	-556.0
	铬离子(Cr⁶⁺)	3CH₄+4Cr₂O₇^2-+32H⁺→8Cr³⁺+CO₂+22H₂O	-878.8
其他类型	硒酸盐(SeO₄^2-)	3CH₄+4SeO₄^2-+8H⁺→4Se+3CO₂+10H₂O	—
	蒽醌‐2,6‐二磺酸盐(AQDS)	CH₄+4AQDS+3H₂O→HCO₃^-+H⁺+4AQH₂DS	-41.0
	高氯酸盐(ClO₄^-)	CH₄+ClO₄^-→HCO₃^-+Cl^-+H⁺+H₂O	-859.9

类型	电子受体	反应式	吉布斯自由能/(kJ/mol)
好氧型	氧气(O₂)	CH₄+2O₂→CO₂+2H₂O	-858.7
硫酸盐还原型	硫酸盐(SO₄²)	CH₄+SO₄^2-→HCO₃^-+HS^-+H₂O	-16.6
反硝化型	硝酸盐(NO₃^-)	CH₄+4NO₃^-→CO₂+4NO₂^-+2H₂O	-483.4
反硝化型	亚硝酸盐(NO₂^-)	3CH₄+8NO₂^-+8H⁺→3CO₂+4N₂+10H₂O	-928.0
金属还原型	铁离子(Fe³⁺)	CH₄+8Fe³⁺+3H₂O→HCO₃^-+8Fe²⁺+9H⁺	-418.3
	锰离子(Mn⁴⁺)	CH₄+4MnO₂+7H⁺→HCO₃^-+4Mn²⁺+5H₂O	-556.0
	铬离子(Cr⁶⁺)	3CH₄+4Cr₂O₇^2-+32H⁺→8Cr³⁺+CO₂+22H₂O	-878.8
其他类型	硒酸盐(SeO₄^2-)	3CH₄+4SeO₄^2-+8H⁺→4Se+3CO₂+10H₂O	—
	蒽醌‐2,6‐二磺酸盐(AQDS)	CH₄+4AQDS+3H₂O→HCO₃^-+H⁺+4AQH₂DS	-41.0
	高氯酸盐(ClO₄^-)	CH₄+ClO₄^-→HCO₃^-+Cl^-+H⁺+H₂O	-859.9

类型	微生物种类		其他
SAMO	ANME-1 ANME-2 ANME-3	ANME-1包含a、b等2个亚簇， ANME-2包含a、b、c、d等4个亚簇	ANME-1主要分布在深海和火山口区域，ANME-2在湿地、湖泊、稻田和垃圾填埋场均有分布，而ANME-3只在极端环境中被检测到
SAMO	SRB	主要包括Desulfosarcina/Desulfococcus（DSS簇）和Desulfobulbus（DBB簇）	ANME-2和ANME-3与SRB耦合紧密，而ANME-1耦合较为松散
DAMO	ANME-2d	包含A、B、C等3个类群	A类由富集培养的古菌组成，在海洋和淡水环境中检测到其序列。 B类和C类分布在极端环境中
DAMO	NC10	包含A、B、C、D、E等5个类群	从富集培养物中鉴定出来的细菌均属于A类，B类和C类经常在自然环境中被检测到，D类在泥炭地表层检测到，E类通过富集培养得到
M-AOM	ANMEs	ANME-1、ANME-3、ANME-2a、ANME-2c、 ANME-2d	几乎所有的ANMEs类群都可以执行此功能
Others	ANME-2	ANME-2d	ANME-2d可以利用不同的电子受体