Abstract: In order to screen degradation of strain to cellulose, more than 217 degrading strains were isolated from the cattle manure of different districts, and test their identification to cellulose through primary screening and secondary screening by Congo red plate method. The XN-13 strain had strong identification capability against the cellulose, and its enzyme activity is 1700U/mL. The strain would have a great value in the research about straw degradation and a good prospect in application. The mycelium shape and colony characteristic observation, a series of physiological and biochemistry experiments and determination of the sequence of 16S rDNA of the fungi had been done. XN-13 was identified as Bacillus subtilis. The obtained of a produce spore cellulose decomposing bacteria XN-13 is very important to industrialization and commodity of inocula.