Abstract:

Abstract: 【OBJIECTIVE】 In order to realize the localization of lily bulbs and breed a great deal of high quality bulbs in China, the author studied the optimal culture media for the inducement of stem tip, multiplication and rooting of Oriental Lily, and meanwhile the author compared the virus-free effect of different culture methods. 【METHOD】The paper investigated the medium optimization of rapid propagation by setting different concentrations of hormone and compared the five kinds of viruses elimination technique of Oriental Lily (Siberia and Sorbonne). 【RESULT】The results showed that the optimal medium of inducing seedlings from stem-tips, sprouts regeneration, seedling growing and rooting was MS (Murashige and Skoog) + 2.0mg/L 6-BA + 0.1mg/L NAA, MS + 1.0mg/L 6-BA + 0.5 mg/L NAA, MS + 1.0mg/L 6-BA + 0.08mg/L NAA and 1/2MS + 0.4mg/L NAA + 4%AC (Active Charcoal), respectively. The virus detection results indicated that the best method of viruses elimination was the stem-tip culture from test-tube seedlings, but the seedling rate was low, and the method of stem-tip culture after Cold Storage is also better in seeding rate and virus-free percent. 【CONCLUSION】The optimal culture media for the different stages of rapid propagation the paper studied met the need of the rapid propagation for Oriental Lily. The result also showed that stem tip culture was a better way for Oriental Lily in the aspect of viruses elimination.