Abstract:

【OBJECTIVE】To investigate the genes regulating biomass and energy in C4 Sugarcane(Saccharum officinarum L). 【METHOD】The ADP/ATP carrier(AAC) gene which is closely related to the energy metabolism was cloned through homology-based cloning , and the sequences was analyzed through the bioinformatics method which analyzes sequence alignment, physical properties, protein tertiary structure, protein subcellular localization, prediction of transmembrane segments and hydrophobicity. 【RESULTS】A 1170bp AAC cDNA sequence was obtained, the sequence contained a 1164 bp ORF coding for a protein of 387 aa. A bioinformatics-based functional analysis shows that the AAC genes of different species are unconserved in N-terminal, but are highly conserved in C terminal. The molecular weigh of ACC is 42.265 kD. The theoretical isoelectric point is 9.79. The possibility of subcellular localization in cytoplasm is 60.9%.The GRAVY is -0.099, is presumed to be a stable protein with six transmembrane segment, and hydrophobicity estimation shows three of the six transmembrane segment may be as the salt bridge to connected the phospholipid bilayer. 【CONCLUSION】In summary, a AAC cDNA with full ORF was cloned, it can be speculated to be a stable protein with transmembrance segments,and location in cytoplasm. These studies aims to provide the theoritical basis of molecular mechanism for AAC regulation in sugarcane.