Abstract:

Explants(cotyledons) was cultured on MS medium supplemented with 1.79 mg/L 2,4-D and 1.15 mg/L Kt for 36 days.And then, Induced callus were subcultured onto the medium with the same mediun plus NaCl in different concentration for salt stress, and the physiological characters (actvities of SOD and POD,contents of soluble protein, MDA and proline) of the callus were tested after0, 3, 6, 9 days of stress. By comparing physiological indexes from different stress, 200mmol/L NaCl was defined as the most suitable salt content for screening mutants resistent to salt stress. Experiments for verification showed that the amount of callus resistent to stress obtained from 200mmol/LNaCl was much more than that from 100mmol/LNaCl stress, while no resistent callus was achieved from 280 mmol/LNaCl stress, confirming the reliability of previous prediction. The results presented here provide the base for obtaining regenerated resistent plants and investigating the resistent mechanism of salt stress of this plant.