Abstract: Abstract: special primer designed and synthesized based on the sequence of the codon of E.coli, and the gene of accB was obtained by PCR amplification and cloned into prokaryotic expression vector pGEX-4T-1. Then the recombinant expression plasmid pGEX-4T-accB was transformed into E. coli BL21 and the fusion protein of accB was produced by IPTG induction. The analysis of SDS-PAGE showed that the fusion pGEX-4T-accB protein was expressed about 43kD.