Abstract:

In this article compared the results of different inactivation and inactivated conditions on the Vibrio ichthyoenteri vaccine. The appropriate method for inactivation the bacterium was treatment the bacteria suspension with 0.4% formalin at 25℃ for 30h. The efficacy of vaccine against Vibrio ichthyoenteri, Vibrio alginolyticus, Vibrio prarhaemolyticus and Vibrio anguillarium on the turbot Scophthalmus maximus L was study by vaccinated with vaccine by immersion and injection routines. The results showed that the relative percent survival (RPS) was 75% for Vibrio ichthyoenteri by injection routines, 67.6% for Vibrio alginolyticus, 50% for Vibrio anguillarium and 57.2% for Vibrio prarhaemolyticu, but the RPS was 25% for Vibrio ichthyoenteri by immersion routines to the turbot, 22.3% for Vibrio alginolyticus, 11.1% for Vibrio anguillarium and 12.5 for Vibrio prarhaemolyticus