Abstract: 【OBJECTIVE】The research aimed to study the propagation and differentiation of PLB of Cymbidium hybridum‘Queen Emeraldus’and C. longibracteatum. 【METHOD】 Four factors of medium, 6-BA, NAA, and additive were designed to study the effects on differentiation and differentiation of PLB of Cymbidium hybridum ×C. longibracteatum .【RESULTS】KC medium was more effective than others. There was no difference with different concentrations of 6-BA. NAA influenced the propagation of PLB directly. NAA0.2mg/L was most favorable to the propagation of PLB. The best differentiation medium was KC+6-BA2.0mg/L+NAA0.5mg/L. 【CONCLUSION】 The medium supplemented with 15mL/L banana was most beneficial to the Propagation of PLB.