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中国农学通报 ›› 2015, Vol. 31 ›› Issue (21): 123-129.doi: 10.11924/j.issn.1000-6850.casb15030025

所属专题: 生物技术 小麦

• 生物技术科学 • 上一篇    下一篇

小麦种子过氧化物酶活性分析

廖金花,郑世刚,吴瑜   

  1. 乐山师范学院生命科学学院,中国科学院成都生物所,中国科学院成都生物所
  • 收稿日期:2015-03-04 修回日期:2015-06-30 接受日期:2015-04-24 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 廖金花
  • 基金资助:
    乐山市科技局重点研究项目“红香米的融合育种”(2011NZD088);中国科学院战略性先导科技专项(A)“高产优质小麦新品种分子设计与培育”(XDA08030106);乐山师范学院项目“川西高原季节性雪被覆盖地区土壤生物和氮循环动态对冬季降雪和凋落物分解的响应”(Z1263)。

Analysis of Peroxidase Activity of Wheat Seed

  • Received:2015-03-04 Revised:2015-06-30 Accepted:2015-04-24 Online:2015-07-28 Published:2015-07-28

摘要: 小麦籽粒过氧化物酶(POD)是和小麦(Triticum aestivum L.)品质密切相关的酶。对种子 POD活性的变异分析无疑为小麦品质改良育种提供理论基础。本研究选用 120个小麦品种(系)为试验材料,用分光光度计法测定了 2年度(2012, 2013)POD活性,并进行了聚类分析(最长距离法)和阳离子活性电泳检测。结果表明,供试小麦品种(系)间的 POD活性均达到极显著差异,年份间 POD活性相关极显著(r=0.5143)。2个年度的品种(系)间差异顺序基本一致,认为 POD活性主要受遗传控制。聚类分析将供试的 120份材料的 POD活性聚为 6类,每类酶活平均值和每类 SDS沉降值大于 4 mL的品种(系)数占每类中总材料数比例之间呈现显著相关性(r=0.939),验证了 POD影响面筋品质的理论。关于电泳,虽然‘KS448’, ‘西昌 177’和 ‘蜀万 831’的 POD 活性都高,但是其同工酶图谱却有明显的差异,推测‘蜀万831’含有的 POD可能主要是阴离子 POD,而 Px3可能主要控制小麦籽粒的颜色和大小。最后这些材料中选择了POD酶活低,面筋品质好,种皮颜色浅的3个品种作为优质小麦品种资源。

关键词: 转Bt, 转Bt, 杂交棉花品种, 苏棉29, 性状

Abstract: Grain peroxidase (POD) activity is closely related to the quality of wheat (Triticum aestivum L.). Studies on the variation of grain POD activity could provide a basis for the genetic improvement of wheat quality. In this study, 120 wheat varieties were used as materials, and the POD activities of two years (2012 and 2013) were determined by spectrophotometry. Furthermore, clustering analysis (Euclidean distance) and cationic active electrophoresis were also used. The results showed that there was a significant variation in the POD activities in the 120 experimental materials, and a highly significant correlation between the POD activities of the varieties in the two cropping seasons (r=0.5143). These findings indicated that POD activity was mainly controlled by genetic factors. With clustering analysis (Euclidean distance), POD might affect the gluten quality as indicated by the relationship (r=0.939). Though the three varieties ( ‘KS448’ ,‘Xichang177’ and‘Shuwan831’ ) had high POD activities, their POD cation isoperoxidase patterns were apparently different. The POD of‘Shuwan831’might be anionic isoenzyme. And isoenzyme Px3 probably controlled the color and size of wheat seeds. Three varieties with low POD activity, strong gluten and light- colored seed coat were selected out of the total number of varieties as high quality variety resources.