小麦腐霉根腐病病原菌鉴定与致病性测定

doi:10.11924/j.issn.1000-6850.casb17060097

中国农学通报 ›› 2017, Vol. 33 ›› Issue (33): 34-38.doi: 10.11924/j.issn.1000-6850.casb17060097

所属专题：小麦

小麦腐霉根腐病病原菌鉴定与致病性测定

张悦丽,张博,马立国,徐作珽,李长松,祁凯,齐军山

山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所,山东省农业科学院植物保护研究所

收稿日期:2017-06-21 修回日期:2017-11-10 接受日期:2017-09-21 出版日期:2017-11-27 发布日期:2017-11-27
通讯作者: 齐军山
基金资助:
山东省自然科学基金面上项目“小麦腐霉根腐病病原及其抗性种质资源研究”(ZR2014CM034)；山东省现代农业产业技术体系小麦创新团队“小麦病虫害防控与质量安全”(SDAIT-01-10)；公益性行业(农业)科研专项经费“小麦根腐病农业及生态防治技术研究”(201503112)；山东省重点研发计划“冬小麦水肥药精准管理关键技术研究”(2016ZDJS08A02)；山东省农业科学院农业科技创新工程“主要农作物病虫害监测与预警” (CXGC2016A09)；山东省农业重大应用技术创新项目“基于生物防治的粮食作物病虫害安全防控技术模式研究与示范”。

Identification and Pathogenicity Detection of the Pathogen Causing Wheat Pythium Root Rot

Received:2017-06-21 Revised:2017-11-10 Accepted:2017-09-21 Online:2017-11-27 Published:2017-11-27

摘要/Abstract

摘要： 本研究旨在探明引起冬小麦苗期生长不良、矮小的原因，并研究明确该病害的病原菌，采用常规组织分离法对小麦水渍状的根系进行分离、纯化，得到2个类似腐霉的分离物SDCY-1和SDJY-1，并对这2个分离物进行形态特征、致病性鉴定及COⅠ序列分析，结果表明，2个分离物菌落白色，菌丝发达，培养基表面有大量的气生菌丝；人工接种后均能引起小麦生长不良、根系变短、鲜重减轻，水渍状至变褐，重新分离后获得的菌株与原接种菌一致。根据形态特征和系统发育树表明2个分离物为同一种腐霉菌—终极腐霉 Pythium ultimum。研究结果可为冬小麦腐霉根腐病防治和抗病育种提供科学依据。

关键词: 多穗石柯, 多穗石柯, 根皮苷, 大孔吸附树脂, 分离纯化

Abstract: This study aims at exploring the causes of poor growth and short and small plant in the seedling stage of winter wheat, and studies the clear pathogens of this disease by using the conventional tissue separation and purification method of the root system of wheat to obtain two similar Pythium isolates, i.e. SDCY-1 and SDJY-1. In addition, the morphological characteristics, identification of pathogenicity and CO I sequence analysis of the results of the two isolates show that the two isolates of the white colonies developed mycelium, and the culture medium surface showed a large number of aerial hyphae. After artificial inoculation, mycelium can cause poor growth, short root, fresh weight reduction, and hygrophanous to brown color changes in wheat. The newly separated strain was similar to that of the original inoculation strain. According to the morphological characteristics and phylogenetic tree, the two isolates were the same species of Pythium, namely Pythium ultimum. The results of this study can provide a scientific basis for Pythium root rot control in winter wheat and resistance breeding.

张悦丽,张博,马立国,徐作珽,李长松,祁凯,齐军山. 小麦腐霉根腐病病原菌鉴定与致病性测定[J]. 中国农学通报, 2017, 33(33): 34-38.

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小麦腐霉根腐病病原菌鉴定与致病性测定

Identification and Pathogenicity Detection of the Pathogen Causing Wheat Pythium Root Rot

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