Abstract: In order to detect the reasons why the Pinusdabeshanensis Cheng et Law is endangered and provide the evidence for the breeding and conservation. The author applied designed the orthogonal test to identify the effect of sucrose, boron, calcium on germination rate and pollen tube length using in vitro pollen germination methods. The results showed that: sucrose was the key factor in its germination and growth followed by boric acid, and calcium chloride played the least role. The ratio of pollen germination could be significantly increased by adding sucrose while it also could be limited when the sucrose was in the high level. Appropriate addition of boric acid could promote pollen germination rather than pollen tube growth. Calcium chloride had limited effect on pollen germination and it seemed that germination rate was not sensitive to the level of calcium chloride. Integrating pollen germination rate and tube growth, the optimum medium for Pinusdabeshanensis Cheng et Law pollen culture should be 4% sucrose+0.05% boric acid+0.025% calcium chloride.