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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 109-112.doi: 10.11924/j.issn.1000-6850.2008-0707

所属专题： 园艺

• 食品 营养 检测 安全 • 上一篇    下一篇

大米、苹果中吡蚜酮残留量测定

唐俊,何成鹏,汤锋,李学德,花日茂,岳永德   

  • 收稿日期:2008-08-18 修回日期:2008-09-16 出版日期:2008-11-05 发布日期:2008-11-05

Study on Analytical Method of pymetrozine Residue

Cheng-peng HE,Feng Tang,Ri-mao Hua,Yong-de Yue   

  • Received:2008-08-18 Revised:2008-09-16 Published:2008-11-05 Online:2008-11-05

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摘要： 建立了吡蚜酮（pymetrozine）在大米和苹果中的残留分析方法。大米样品以乙腈+氨水（10：1，v:v）提取，苹果样品用乙腈提取，提取液经固相萃取小柱净化，高效液相色谱(VWD)测定。吡蚜酮的最小检测量为6.0×10-10g，最低检测浓度为0.02mg/kg。大米中吡蚜酮的添加（浓度0.05～1.0mg•kg-1）回收率为75.99%～96.03%，变异系数分别为1.64%～4.74%；苹果中吡蚜酮的添加（浓度为0.05～1.0 mg•kg-1）回收率为74.84%～86.28%,，变异系数分别为1.67%～5.43%。该方法的准确性和灵敏度均符合农药残留分析要求。

关键词: 磨盘柿, 磨盘柿, 叶片, 果实, 酚类物质

Abstract: High performance liquid chromatography was used to analysis pymetrozine residues in rice and apple.Residues of pymetrozine were extracted from rice with acetonitrile:ammonia(10:1,V:V) and apple with acetonitrile,cleaned up by SPE column,and determined by HPLC equipped with VWD.The average recoveries and coefficient of variations of the method were 75.99%～96.03%, 1.64%～4.74% for rice sample and 74.84%～86.28%, 1.67%～5.43% for apple sample.The minium detectable amount and concentration of pymetrozine were 6×10-10g and 0.02 mg•kg-1.The method of pymetrozine in rice and apple accords with demands of residue analysis of pesticide.