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中国农学通报 ›› 2009, Vol. 25 ›› Issue (6): 37-39.

所属专题: 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

新城疫病毒诱导黄粉虫抗病毒肽的效果分析

王立新,路振香,王金虎,王珊珊,胡静,戴四发   

  • 收稿日期:2008-11-21 修回日期:2009-02-10 出版日期:2009-03-20 发布日期:2009-03-20

The Effect of Tenebrio Antiviral Peptide Induced By Newcastle Disease Virus

  • Received:2008-11-21 Revised:2009-02-10 Online:2009-03-20 Published:2009-03-20

摘要: 目的:观察鸡新城疫病毒(NDV)诱导黄粉虫是否产生抗鸡新城疫病毒肽。方法:先用鸡胚培养鸡新城疫病毒以扩增病毒,然后提取病毒液备用,接着用鸡新城疫病毒液接种刺伤黄粉虫幼虫,2~3天后提取黄粉虫体内的抗病毒物质,测定其血凝效价和鸡新城疫病毒诱导鸡胚发病抑制试验结果。结果 :黄粉虫抗病毒肽在血凝抑制试验中的效价为1∶64;在鸡新城疫病毒诱导鸡胚发病抑制试验中有一定效果,但不明显。

关键词: 肥料, 肥料, 修剪高度, 果岭, 影响

Abstract: The experiment was conducted to observe inhibition of anti-viral substances in Tenebrio Molitor induced by Newcastle disease virus (NDV) to Newcastle disease virus. Chook embryo was used to culture Newcastle disease virus to enlarge its amount, and then NDV liquid was extracted from it, and Tenebrio Molitor larvae was stabbed by needle containing NDV, after 2 to 3 days anti-viral substances was picked up from Tenebrio Molitor larvae. The blood coagulation capability of anti-viral substances was confirmed and the experiment that chook embryo was induced to bring on of a disease was done. The results reveal that The blood coagulation capability of anti-viral substances was 1∶64. The effect in inhibition of NDV induction chook embryo to disease has certain, but it was not obvious.