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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (1): 223-228.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

南方根结线虫延长因子2基因cDNA全长克隆和序列分析

黎娟华 赵平娟 孙海彦 彭 明   

  • 收稿日期:2010-06-29 修回日期:2010-09-08 出版日期:2011-01-07 发布日期:2011-01-07
  • 基金资助:

    中央级公益性科研院所基本科研业务费专项项目（ITBBZX0812）“利用 RNAi 技术防治南方根结线虫的研究”

Cloning and Sequence Analysis of Full-length cDNA of Elongation Factor 2 Gene of Meloidogyn incognita

  • Received:2010-06-29 Revised:2010-09-08 Online:2011-01-07 Published:2011-01-07

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摘要：

以南方根结线虫为材料，根据同源序列法分别进行3′末端和5′末端的RACE，获得延长因子基因2 cDNA全长。此cDNA全长序列为2434 bp，包括2334 bp的完整ORF，编码一含777个氨基酸的多肽。该基因命名为Mi-eft2，其推导蛋白MI-EFT2的编码氨基酸与秀丽隐杆线虫的Ce-eft1，Ce-eft2，Ce-eft3，Ce-eft4基因的推导蛋白的同源性分别为26.7%、86.9%、13.8%和8.9%。蛋白功能分类预测MI-EFT2是一种在蛋白质延长过程中起作用的GTP水解酶，与真核生物的延长因子2功能相同。

关键词: 朱蕉, 朱蕉, RAPD, 形态性状, 聚类分析

Abstract:

The full-length cDNA sequence of elongation factor 2 (Mi-eft2) was obtained by RT-PCR and RACE from Meloidogyne incognita. It consisted of 2434 nucleotides, and encoded 777 amino acids. The amino acid sequence of deduced protein MI-EFT2 was 26.7%, 86.9 %, 13.8% and 8.9% identical with the deduced proteins of Ce-eft1, Ce-eft2, Ce-eft3 and Ce-eft4 from Caenorhabditis elegans respectively. Protein function prediction of MI-EFT2 suggested that it was a GTPase just like the eukaryotic elongation factor EF2, involved in the protein elongation process.

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