欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (19): 23-27.

• 林学 园艺 园林 • 上一篇    下一篇

银杏叶片RNA提取方法的研究

尤超 赵大球 梁乘榜 周春华   

  • 收稿日期:2011-01-30 修回日期:2011-02-15 出版日期:2011-08-05 发布日期:2011-08-05
  • 基金资助:

    江苏省自然科学基金;江苏省教育厅高校自然科学基金;扬州大学科技创新培育基金;扬州大学科技创新培育基金

Comparison of Methods for Total RNA Extraction from Ginkgo biloba L.

  • Received:2011-01-30 Revised:2011-02-15 Online:2011-08-05 Published:2011-08-05

PDF (PC)

1341

摘要：

为了获得一种银杏（Ginkgo biloba L.）叶片总RNA提取的理想方法，为后续的分子生物学研究打下基础，笔者以不同生长期的叶片为材料，利用生物分光光度计和凝胶电泳法比较了改良CTAB法、乙二醇丁醚法以及改良Trizol法提取的银杏总RNA的产率和纯度。结果表明：改良Trizol法所提取RNA的OD260/OD280比值介于1.8~2.0之间，并且OD260/OD230在1.9~2.1之间，具有较高的纯度。凝胶电泳结果表明，改良Trizol法有28S rRNA和18S rRNA 2条清晰的条带，且很少有降解；CTAB法获得的RNA品质也较好，但存在降解和弥散现象，乙二醇丁醚法提取效果较差。这表明改良Trizol法提取的总RNA具有很高的纯度，可以满足进一步分子生物学研究的要求。

关键词: 褐飞虱, 褐飞虱, 种群数量, 运动周期, 变化规律, 动态模型

Abstract:

In order to obtain an ideal total RNA extraction method of Ginkgo biloba L. providing foundation for molecular biology research, total RNA from leaves at different growth stages were extracted with three methods, including CTAB method, ethylene glycol monobutyl ether method and a modified Trizol method, and its quality and quantity were compared. The results indicated that the modified Trizol method was better than the other methods since the value of OD260/OD280 and OD260/OD230 were 1.8-2.0 and 1.9-2.1, the purities were higher, the bands of 28S and 18S rRNA on gel electrophoresis were very clear and did not degradated. Comparably, CTAB method was not very good as the first method because RNA was easy degraded and dispersed although the value is higher, and ethylene glycol monobutyl ether method was not fit the requirement of RNA extraction.

中图分类号: