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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (16): 277-281.doi: 10.11924/j.issn.1000-6850.2011-2639

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

辣椒杂交种纯度快速鉴定体系建立与应用分析

白占兵 李雪峰 谭新跃 戴雄泽 马艳青 张竹青 陈文超   

  • 收稿日期:2011-09-13 修回日期:2012-02-17 出版日期:2012-06-05 发布日期:2012-06-05

Rapid Identification of Hybrid Pepper Purity Establishment and Application System

  • Received:2011-09-13 Revised:2012-02-17 Online:2012-06-05 Published:2012-06-05

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摘要：

为了解决辣椒杂交种快速鉴定的问题，有必要建立辣椒杂交种的室内纯度鉴定体系，以兴蔬种业的32个品种的父本、母本和F1共96份为试验材料，利用150对SSR引物对96份材料进行了PCR扩增，寻找扩增差异。扩增结果发现，150对引物中51对能扩增出清晰条带，19对多态性较好，5对引物能够区别兴蔬种业32个品种的父本、母本及其F1。SSR分子标记能够快速准确地鉴定辣椒杂交种纯度与田间鉴定结果一致且成本低廉。

关键词: 覆盖度, 覆盖度, 图像处理, 叶面积指数（LAI）, 模型

Abstract:

In order to solve the problem of Capsicum hybrid rapid identification of the problem, it is necessary to establish the hot pepper hybrid seed purity identification system indoor, 96 (Xingshu seeds 32 varieties of male, female and F1) as the test material, using 150 pairs of SSR primers and 96 materials were amplified by PCR, looking for the amplification of discrepancy. The amplification results found in 51 on 150 pairs of primers can amplify clear bands, 19 pairs of polymorphism was better, 5 pairs of primers can distinguish Xingshu seed 32 varieties of male, female and their F1. SSR markers can be quickly and accurately identify the purity to pepper hybrids were consistent with field identification, and low cost.