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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (11): 20-23.doi: 10.11924/j.issn.1000-6850.2011-3259

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

中介蝮蛇消化道5羟色胺细胞的免疫组织化学定位

张珈宁 张欣 李淑兰   

  • 收稿日期:2011-11-07 修回日期:2011-11-18 出版日期:2012-04-15 发布日期:2012-04-15
  • 基金资助:

    哈尔滨师范大学第五届大学生实践创新基金课题“中介蝮消化道5羟色胺细胞的免疫组织化学定位”;2009 年校科技发展预研项目“极北鲵发育形态学和免疫组织化学研究”

Immunohistochemical Localization on 5-HT Cells in the Digestive Tract of Gloydius intermedius

  • Received:2011-11-07 Revised:2011-11-18 Online:2012-04-15 Published:2012-04-15

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摘要：

为了研究中介蝮蛇（Gloydius intermedius）消化道5-羟色胺（5-hydroxytryptamine，5-HT）细胞的形态和分布规律。应用免疫组织化学ABC法。结果表明，5-HT细胞在其整个消化道中均有分布。其中以胃幽门部分布密度最高，直肠部分布密度最低。在整个消化道中，均可见到呈圆形或椭圆形的闭合型5-HT细胞；开放型5-HT细胞呈蝌蚪形、锥体形，集中分布于食管、贲门、幽门和小肠各段。表明5-HT细胞分布的特点可能与中介蝮蛇的食性、生活环境或消化道各部位的功能有关。

关键词: 施N策略, 施N策略, 膜下滴灌, 产量, SPAD值, 碱解氮

Abstract:

To study the morphology and density of 5-hydroxytryptamine (5-HT) cells in the digestive tract of the Gloydius intermediu. The method of the ABC immunohistochemical technique. The results showed that the 5-HT cells could be observed throughout the digestive tract with the highest density in the gastric pylorus and the lowest density in the rectum. What could be seen throughout the whole digestive tract was round or oval of closed-type 5-HTcells; the open-type 5-HT cells were tadpole-shaped or cone-shaped and mainly located in the esophagus, gastric cardia, gastric pylorus and sections of small intestine. The distribution characteristics of 5-HT cell may be related to the feeding habits and the living environment or function of various parts of the digestive tract. Key words: Gloydius intermedius; digestive tract; 5-HT cells

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